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- When all imaging parameters are set up press the XY button to start the image acquisition.
When the image acquisition has finished a new window with the recorded image file will open. To save the image highlight it, go to file Save as…, enter a filename and click save. Files are best saved as .oif or .oib, which are Olympus’ own file formats containing all acquisition metadata. You should save all data on the PC’s D: drive when acquiring and later copy them to a safe location such as your project folder on the UCL Active Directory. Instructions how to connect to your project folder can be found here.
Info OIB is the newer Olympus file format. In contrast to OIF, where image data is stored as many separate TIFF files with an OIF database file as an index, the OIB file stores all data in one file. Some offline image processing software might not be compatible with OIB. Volocity, for example, will only read OIF files.
Simultaneous and sequential acquisition
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