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FV1200 Software - Quick Guide

This PDF Quick Guide shows the FV10-ASW 4.2 software user interface and summarises the key features required for basic image acquisition. It prints out onto an A4 sheet for handy reference.

Starting up the microscope

Turn on the Fluorescent Lamp wall socket switch to the left of the air table.
Press the ON/OFF button on the front of the U-HGLGPS fluorescent lamp beneath the air table. It is on when the blue Burner Status LED comes on.
Check that the Computer and Monitor socket switches are switched on at the wall and start the computer up by pressing the main power button
The pGina authentication screen takes the place of the normal Windows login. Wait until its service status changes to Connected and then login with your Agendo booking system credentials.
Switch on the wall sockets labelled Power Controller 1 and Power Controller 2. These will power up most of the microscope devices. Do not use any of the switches on the power controllers themselves; these should always remain switched on.
Turn on the I3-TPC Touch Panel Controller by pressing the power button on its back (see arrow in panel B below).
Press Start Operation on the I3-TPC touch screen.
Most of the laser power packs are on the shelf above the microscope. The main power switches of the lasers are always in the ON position.
Turn the Argon laser key to ON.
The 559 laser Opti λ power pack receives mains power when Power Controllers 1 and 2 are turned on, at which point the TEMP LED will start flashing (A below). The LED will continue to flash for 5 - 10 min. Once it stops, turn the LASER key to ON and wait for a few minutes for the two red LEDs labeled LASER to stop flashing (B below). Do not open the confocal microscope software until these stop flashing.
If you would like to use the 440 nm laser to excite CFP and other cyan dyes you will need to turn on the FV5-LDPSU controller key, otherwise this does not have to be turned on.
If you need to use the pulsed PicoQuant 405 nm laser for ablation experiments turn the key on the PDL 800-D controller on the shelf from STBY to ON, otherwise this can stay in the STBY position.
Double-click the FV10-ASW 4.2 icon on the Desktop to open the software. Select the Guest user ID in the 'Welcome to FV10-ASW' dialog and click OK. There is no password for this account.
The software will take some time to start during which 'Hardware Initializing…' will be displayed on the computer screen and the touch panel controller will say 'Connected software is sending information to this device'.
Allow the lasers at least 30 min to warm up before you start imaging. You are now ready to put a specimen on the microscope and set up your image acquisition.