...
- Set the Offset as described above
- Press Continuous AF in the ZDC Control panel
- The ZDC locks onto the AF plane, but this won't necessarily be the plane you are interested in
- Scan your specimen with Continuous AF on
- Press the Continuous AF tab on the I3-TPC touch pad controller
- The yellow line (red arrow below) indicates the position of the Focus Limit
- Press the Fine button on the controller to activate the fine focus
- Adjust the focus until the plane of interest is in focus
- Press the Fine button again to reactivate the Continuous AF at this new position
- Start your imaging (AF status will show “Focussing” throughout the entire image acquisition)
...