Technical information and user guides

Sample preparation guide from 3i

Quick alignment guide from 3i

Image processing guide from 3i

SlideBook software manual for diSPIM

10x 0.3 NA water lens configuration

40x 0.8 NA water lens configuration

16.7x Special Optics 54-10-12 multi-immersion lens configuration

The diSPIM (aka 3i Marianas LightSheet) is a dual inverted Selective Plane Illumination Microscope in which the sample can be imaged flat on a standard microscope stage. This contrasts with light sheet configurations such as the LightSheet Z.1 in which the sample must be mounted in an imaging chamber, which can require more complex sample preparation steps.

Here is a list of some example research papers in which diSPIM systems have been used for imaging biological specimens.

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