Leica TCS SPE inverted confocal microscope

Access & Usage

Training

Confocal Booking System (Faces Scheduling System)

Microscope Booking Rules

Technical Specifications

Microscope Components (Objective lenses, filter sets, lasers, etc)

User Guides

SPE3 Quick Start

Using the buttons and other controls on the DMI4000 microscope for fluorescence, bright-field or DIC

Transmitted Light DIC on SPE3

Confocal Resolution Calculator

Correcting chromatic shift using Volocity

Location: MRC Building, Room 2.16

The SPE3 is a 2012 model Leica TCS SPE confocal microscope mounted on a semi-automated, inverted Leica DMI4000 stand. The stand is capable of fluorescence, bright-field and DIC techniques and is fitted with a motorised stage. The confocal system has four lasers and a single photomultiplier tube (PMT) detector so multichannel acquisition must be done sequentially. The microscope stand has a manual focus drive so optical sectioning is achieved using the Z Galvo insert integrated into the stage. The software has Tile Scan and Mark & Find modes, which use the microscope's motorised stage to revisit saved positions or build up a 'mosaic' of images of adjacent areas.

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