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Ultraview Vox Lasers and Fluorescence Filters

Ultraview Vox Lasers and Fluorescence Filters

May 06, 2016

Lasers

The Vox has diode or DPSS lasers that emit the following wavelengths: 405 nm, 488 nm, 561 nm and 640 nm.

Emission filters in the filter wheel

These filters are used when you are imaging. The filter in position 7 is a dual band pass filter letting through green and red fluorophores. It is used in Fast Sequential mode. All of the filters are selectable in Volocity when you are in Emission Discrimination mode.

Turret Position

Emission Filter (nm)

Example Fluorophores

Notes

1

Empty

N/A

 

2

527(55)

GFP

Use this for green fluorescence in Emission Discrimination mode

3

455(80) 615(70)

DAPI, Texas Red

Use this for blue and red fluorescence in Emission Discrimination mode

4

485(60) 705(90)

CFP, Cy5

Use this for far red fluorescence in Emission Discrimination mode

5

587(125)

YFP

 

6

DIC

Brightfield DIC

Use this analyser when imaging DIC 

7

525(50) 640(120)

GFP, RFP

This filter is automatically selected in Fast Sequential mode

8

477(45) 575(100) 705(90)

Hoechst, YFP, Cy5

 

9

Empty

N/A

 

10

Empty

N/A

 

Note that although there are filters for CFP and YFP the confocal system does not have lasers at 440 nm or 515 nm that would be optimal for exciting these fluorophores, although the 488 laser can be used to excite YFP.

Filter cubes in the microscope

These filters are used when viewing your specimen down the microscope eyepieces. You can select them using the microscope Remote Control Pad or in Volocity. The filter turret must be in position 1 (EMPTY) when imaging with the spinning disc.

Position Number

Filter Set

Excitation Filter

Dichroic Mirror

Emission Filter

Example Fluorophores

1

EMPTY

N/A

N/A

N/A

None. For use with bright field or when imaging with the spinning disc

2

DAPI

D350/50x

400DCLP

D460/50m

DAPI/Hoechst/AMCA

3

FITC

HQ470/40x

Q495LP

HQ525/50m

Endow GFP

4

TRITC

HQ575/50x

Q610LP

HQ640/50m

HcRed1, mCherry

5

ANALY

N/A

N/A

N/A

DIC analyser (for viewing DIC in eyepieces)

6

EMPTY

N/A

N/A

N/A

None. Do not use.