GFP1 Objective Lenses and Filters

GFP1 - Objective Lenses and Fluorescence Filters

Objective Lenses on the Axiovert 200M Microscope

Magnification	Immersion Medium	Designation	Numerical Aperture	Coverslip Correction	Contrast Technique	Working Distance (mm)
10X	Air	Plan Neofluar	0.3	0.17	Ph1	5.2
20X	Air	Plan Neofluar	0.5	0.17	Ph2	2
20X	Air	LD Achroplan	0.4	0 - 1.5	Ph2	10.2
40X	Air	LD Plan Neofluar	0.6	0 - 1.5	Ph2	2.9
63X	Oil	Plan Neofluar	1.25	0.17	Ph3	0.1

Other objective lenses are available, including high resolution oil objectives. Phase contrast using the Ph1 and Ph2 objectives above will work fine with the 0.35 NA long distance condenser, but high resolution oil objectives that require Ph3 would need a 0.55 NA condenser.

Fluorescence Filter Sets on the Zeiss Axiovert 200M Microscope

Turret Position	Filter Set	Excitation Filter	Dichroic Mirror	Emission Filter	Example Fluorophores
1	Zeiss FS02	G365	FT 395	LP 470	DAPI
2	GFP-3035D	472/30	FF495-Di03	520/35	GFP
3	Cy3 41002b	HQ545/30	Q570lp	HQ610/75m	Cy3
4	TxRed-4040C	562/40	FF593-Di03	624/40	RFP
5	Cy5 49006	620/60	t660lpxr	700/75	Cy5