GFP2 Objective Lenses and Filters
GFP2 - Objective Lenses and Fluorescence Filters
Objective Lenses on the Axiovert 200M Microscope
Magnification | Immersion Medium | Designation | Numerical Aperture | Coverslip Correction | Contrast Technique | Working Distance (mm) |
|---|---|---|---|---|---|---|
4X | Air | Achroplan | 0.1 | None | None | 11.1 |
10X | Air | EC Plan Neofluar | 0.3 | 0.17 | Ph1 | 5.2 |
20X | Air | LD Achroplan | 0.4 | 0 - 1.5 | Ph2 | 7.9 |
40X | Air | LD Plan Neofluar | 0.6 | 0 - 1.5 | Ph2 | 2.9 |
Other objective lenses are available, including high resolution oil objectives. Phase contrast using the Ph1 and Ph2 objectives above will work fine with the 0.35 NA long distance condenser, but high resolution oil objectives that require Ph3 will need the 0.55 NA condenser.
Fluorescence Filter Sets on the Zeiss Axiovert 200M Microscope
Turret Position | Filter Set | Excitation Filter | Dichroic Mirror | Emission Filter | Example Fluorophores |
|---|---|---|---|---|---|
1 | Zeiss Filter Set 05 | BP395-440 | FT460 | LP470 | eCFP |
2 | Zeiss Filter Set 09 | BP 450-490 | FT510 | LP515 | FITC |
3 | Semrock Texas Red | 562/40 | 601 | 624/40 | mRFP1 |
4 | Semrock eGFP | 472/30 | 495 | 520/35 | eGFP |
5 | None | N/A | N/A | N/A | Transmitted Light |