SPE System Information

DM2500 Microscope

The SPE confocals are built around manual DM2500 microscope stands. The fluorescence filter turret and objective lens nosepiece are manually rotated. There is no internal focus motor; confocal sectioning is achieved via a galvanometer driven stage insert. Eyepiece or confocal light path selection is via manual slider. DIC components are available for these microscopes.

Objective lenses on the Leica DM2500 Microscope

Magnification

Immersion Medium

Designation

Numerical Aperture

Coverslip Correction

DIC Prism

10x

Air

ACS APO

0.3

None

C

20x

Multi

ACS APO

0.6

0 - 0.17

 

40x

Oil

ACS APO

1.15

0.17

E

63x

Oil

ACS APO

1.3

0.17

E

*63x

Water Dipping

HCX APO

0.9

0

 

**40x

Water Dipping

HCX APO

0.8

0

 

**20x

Water Dipping

HCX APO

0.5

0

 

*Objective only available on the SPE not the SPE2
**Objective only available on the SPE2 not the SPE

Fluorescence filter sets on the Leica DM2500 microscope

Turret Position

Filter Set

Excitation Filter

Dichroic Mirror

Emission Filter

Example Fluorophores

1

N2.1

BP515-560

RKP580

LP590

Texas Red

2

I3

BP450-490

RKP510

LP515

FITC/GFP

3

A

BP340-380

RKP400

LP425

Hoechst/DAPI

4

BF

N/A

N/A

N/A

Transmitted Light/Confocal

5

*B/G/R

BP 420/30; 495/15; 570/20

415; 510; 590

BP 465/20; 530/30; 640/40

Blue, Green and Red

*B/G/R is not currently installed on SPE2