SP5 Microscope Information

Owned by Andrew Vaughan
Last updated: Jun 17, 2014Version comment
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Leica DMI6000 Microscope

Objective Lenses on the Leica DMI6000 Microscope

Magnification

Immersion Medium

Designation

Numerical Aperture

Coverslip Correction (mm)

Working Distance (mm)

10X

Air

HC PL FLUOTAR

0.3

None

11

20X

Multi

HC PL APO

0.7

0 - 0.17

0.26

40X

Oil

HCX PL APO

0.75 - 1.25

0.17

0.1

63X

Oil

HCX PL APO

0.6 - 1.4

0.17

0.1

*63X

Glycerol 21°C

HCX PL APO

0.75 - 1.25

0.14 - 0.18

0.28

*40X

Air

HCX PL FLUOTAR

0.75

0.17

0.4

* These objective lenses are available but aren't routinely fitted to the microscope

Fluorescence Filter Sets on the Leica DMI6000 Microscope

Turret Position

Filter Set

Excitation Filter

Dichroic Mirror

Emission Filter

Example Fluorophores

1

I3

BP450-490

RKP510

LP515

FITC/GFP

2

N2.1

BP515-560

RKP580

LP590

Texas Red

3

Pol

N/A

N/A

N/A

Polariser for DIC and polarised light microscopy

4

BF

N/A

N/A

N/A

Empty for transmitted light and confocal scanning

5

A

BP340-380

RKP400

LP425

Hoechst/DAPI

6

CFP

BP 436/20

455

BP 480/40

CFP

The filter positions above control what the user sees down the microscope while preparing his/her sample and not what light is acquired during a confocal scan.

Buttons 3 and 4 are not active; the transmitted light illumination mode is controlled by the TL/IL and ChTL buttons.