Super-Resolution Microscopy

Sample Preparation

Gatta-STORM 94R

1. Wash one of the central two wells of the Ibidi μSlide chamber three times with 700 μl PBS.
2. Incubate the chamber with 500 μl of BSA-biotin solution (1 mg/ml in PBS) for 5 min.
3. Remove the BSA-biotin solution and wash it three times with 700 μl PBS. Make sure not to scratch the chamber surface. Pick one corner and always pipette at that position.
4. Incubate the chamber with 500 μl of neutravidin solution (1 mg/ml in PBS) for 5 minutes.
5. Remove the neutravidin solution and wash the chamber three times with 700 μl of immobilisation buffer (IB).
6. Dilute 3 μl of GATTA-STORM DNA origami solution in 500 μl of IB and incubate the chamber with this solution for 5 min.
7. Wash the chamber three times with 700 μl IB.
8. Fill the chamber with the required amount of GLOX switching buffer, supplemented with 10 mM MgCl₂.

Glox Switching Buffer

The Glox buffer formulation is adapted from the following publication:

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Dispense into 400 µl aliquots and store at -20°C.

Solution C - Gatta-STORM version (Reducing Agent Solution)

• 113.6 mg MEA-HCl
• 1 ml distilled water with 100 mg/ml MgCl₂

Use fresh or make 100 µl aliquots for storage at -20°C

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