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Bitplane Learning Zone (includes video tutorials)

Huygens

Licence: Huygens Essential wide-field, confocal and spinning disc on WOPR computer. Huygens Professional confocal and STED on STED and Orac computers

Operating Systems: Windows (but can also run on Mac OS and Linux)

Key features: Deconvolution, but also has tools for 3D reconstruction and visualisation.

Scientific Volume Imaging's Huygens is a modular deconvolution software package. It has a core program that can be used to open, visualise and export data but additional modules must be installed to deconvolve wide-field, confocal, spinning disc and STED data. Multi-photon data can also be deconvolved but we don't have this module yet. We have Huygens on three machines but the licences vary, so you may not be able to deconvolve your data on all the machines (e.g. wide-field data can only be deconvolved on WOPR, confocal data can be deconvolved on all three machines). Additional modules for 3D rendering and image analysis are available but are not installed on our systems so Huygens is mostly used for deconvolution. Huygens is my preferred deconvolution package because it handles low signal-to-noise ratio images well, it is easy to edit parameters in the deconvolution routine and SVI provides a lot of information on their website. You have to register with SVI and get a login to access much of the content on the website.

Website:

Scientific Volume Imaging homepage

SVI's YouTube channel - video tutorials

Analysis Computers

Beast

Processor: 2x Intel XEON CPU X5650 2.67 GHz, 6 cores, 12 logical processors

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