LocationRoom 1.19, 1st floor, MRC Building DescriptionUpright Axioplan II research fluorescence microscope suitable for fluorescence and transmitted light differential interference contrast (DIC) imaging of samples that have been fixed and mounted on slides. The 25x objective can also be used for water dipping provided the dish is wide enough to accommodate the lens. The microscope has a QImaging QIClick greyscale CCD camera for high sensitivity fluorescence imaging. The Apple Mac computer currently has Openlab and Micro-Manager installed for image acquisition. Objective LensesMagnification | Immersion | Designation | NA | Contrast | Coverslip Correction (mm) | WD (mm) |
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5X | Air | Fluar | 0.25 | None | 0.17 | 12.5 | 10X | Air | EC Plan Neofluar | 0.3None | 0.17 | 5.2 | 25X | Multi | Plan Neofluar | 0.8DIC | 0 - 0.170.21 | 40X | Oil | Plan Neofluar | 1.3 | None | 0.170.2 | 63X | Oil | Plan Apochromat | 1.4None | 0.170.18 | 100X | Oil | Plan Neofluar | 1.3 | None | 0.2 |
All lenses are corrected for #1.5 coverslips (0.17 |