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Use a widefield microscope if your specimen is thin and relatively free of out-of focus blur or you don't need to optically section it for processing, analysis and visualisation in 3D.
Microscope Name | Microscope Model | Upright or Inverted? | Objective Lenses | Fluorescence ColoursCamera | Automation | Techniques | |
|---|---|---|---|---|---|---|---|
Axioplan | Zeiss Axioplan 2Upright | 10x air to 100x oil | Blue, Green, Red, Far-Red | Greyscale high sensitivity CCD | Manual | Zeiss Axioplan 2 stand. Good for high sensitivity and high resolution fluorescence microscopy and differential interference contrast (DIC) of fixed specimens on slidesAxio Imager | |
Axio Imager | A1Upright | 10x air to 100x oil | Blue, Green, Red, Far-Red (Cyan and Yellow available on request) | Greyscale high sensitivity CCD | Manual | Zeiss Axio Imager A1 stand. Good for high sensitivity and high resolution fluorescence microscopy and phase contrast of fixed specimens on slides | |
Axioskop 3 | Zeiss AxioskopUpright | 10x air to 100x oil | Blue, Green, Red | Colour CCD | Motorised | Zeiss Axioskop 3 stand. Good for moderate to high resolution fluorescence microscopy and colour imaging of histologically or histochemically stained specimens on slides. Supports tiling and stitching. Colour camera and motorised stage. | |
| Leica DMIRB | Inverted | 10x air to 40x LWD* | Blue, Green, Red | Greyscale high sensitivity CCD | Manual | Leica DMIRB stand. Good for moderate resolution fluorescence microscopy and phase contrast of fixed/live specimens on slides or in dishes and plates with glass or plastic bottoms | |
Leica 2 | Leica DMIRB | Inverted | 10x air to 40x LWD* | Blue, Green, Red | Colour CCD | Manual | Leica DMIRB stand. Good for moderate resolution fluorescence microscopy and colour imaging of histologically or histochemically stained specimens on slides or in dishes and plates with glass or plastic bottoms |
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