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Microscope Name | Upright or Inverted? | Objective Lenses | Fluorescence Colours | Techniques |
|---|---|---|---|---|
Axioplan | Upright for slides | Up to 100x oil | Blue, Green, Red, Far-Red | Zeiss Axioplan 2 microscope with QImaging QClick camera for high sensitivity and high resolution fluorescence microscopy and differential interference contrast (DIC) of fixed specimens on slides |
Axio Imager | Upright for slides | Up to 100x oil | Blue, Green, Red, Far-Red (Cyan and Yellow available on request) | Zeiss Axio Imager A1 microscope with QImaging Retiga EXi for high sensitivity and high resolution fluorescence microscopy and phase contrast of fixed specimens on slides |
Axioskop 3 | Upright for slides | Up to 100x oil | Blue, Green, Red | Zeiss Axioskop microscope with QImaging QICAM colour camera for moderate to high resolution fluorescence microscopy and imaging of histologically or histochemically stained specimens on slides. Motorised stage supports tiling and stitching of specimens. |
Inverted for TC dishes | Long distance air lenses up to 40x | Blue, Green, Red | Leica DMIRB microscope with Hamamatsu Orca NRB camera. Good for moderate resolution fluorescence microscopy and phase contrast of fixed/live specimens on slides, or in dishes and plates with glass or plastic bottoms | |
Leica 2 | Inverted for TC dishes | Long distance air lenses up to 40x | Blue, Green, Red | Leica DMIRB microscope with Leica DFC7000T colour camera for high resolution fluorescence microscopy and colour imaging of histologically or histochemically stained specimens on slides, or in dishes and plates with glass or plastic bottoms |
Confocal Microscopes
Use a confocal microscope if your specimen in thick, has significant out-of-focus blur, or if you need to process, analyse or visualise it in 3D.
Microscope Name | Microscope Model | Upright or Inverted? | Objective Lenses | Lasers | Detectors | Simultaneous/Sequential | Manual/Automated Stage | Climate Environmental Control | Techniques | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
Radiance | Bio-Rad Microscience Radiance 2100 with Nikon E800 stand | Upright | 10x air to 60x Upright for slides | Up to 40x oil (water dipping lenses available) | Argon, 543, 635 | 3x GaAsP | Simultaneous and Sequential | Manual | Available (heat only) | Imaging Heated stage adapter and lens heater | Bio-Rad Microscience Radiance 2100 with Nikon 800 stand for imaging of specimens on slides and in gasket sealed chambers or open dishes using dipping lenses. Three high sensitivity GaAsP detectors. DIC. FRAP. |
SPE | Leica TCS SPE with DM2500 stand | Upright | 10x air Upright for slides | Up to 63x oil (water dipping lenses available) | 405, 488, 532, 640 | 1x PMT | Sequential only | Manual | Available | Imaging of specimens on slides and live specimens in Oko-Lab stage adapter or Warner perfusion chamber | Leica TCS SPE with DM2500 stand for imaging of specimens on slides. Live imaging possible with water dipping lenses using Oko-lab stage adapter or Warner Instruments perfusion chamber using . DIC (not dipping lenses). DIC. Spectral imaging. |
SPE | Leica TCS SPE with DM2500 stand | Upright | 10x air to 63x oil (water dipping lenses available) | 405, 488, 532, 640 | 1x PMT | Sequential only | Manual | Available | Temperature and CO2 | Imaging of specimens on slides and live specimens in open dishes using Oko-Lab incubation equipment and dipping lenses. DIC. . Live imaging possible with water dipping lenses using Oko-lab stage adapter or Warner Instruments perfusion chamber. DIC (not dipping lenses). Spectral imaging. | |
SPE3 | Leica TCS SPE version 2 with DMI4000 stand | Inverted | 10x air to 63x oil | 405, 488, 561, 640 | 1x PMT | Sequential only | Automated | No | Imaging of specimens on slides and in dishes. DIC. Spectral Imaging. Multi-point and stitching. Matrix Screener. Motorised stage. | ||
SP5 | Leica TCS SP5 with DMI6000B stand | Inverted | 10x air to 63x oil (63x glycerol available) | 405, Argon, 561, 594, 6331x PMT and 3x Hybrids | Simultaneous and Sequential | Automated | Available | Imaging of specimens on slides and in 35 mm dishes and Lab-Tek chambered coverslips using Pe-Con incubation equipment. Spectral imaging. DIC. Multi-point and stitching. FRAP. FRET. Live Data Mode. Matrix Screener. | |||
Vox | Perkin Elmer Ultraview Vox with Nikon TiE standInverted | 10x air to 100x oil (60x water immersion available) | 405, 488, 561, 640 | 1x EMCCD camera | Sequential only | Automated | Available | Imaging of live specimens in 35 mm dishes, Lab-Tek chambered coverslips and Ibidi uSlides using Solent Scientific cage type incubation chamber. DIC. Multi-point and stitching. FRAP. | |||
| 3iYokogawa CSU-W1 with Zeiss Axio Observer Z1 stand | Inverted | 10x air to 100x oil (40x water immersion available) | 405, 440, 488, 514, 561, 640 | 1x EMCCD camera and 1x sCMOS camera | Sequential only | Automated | Available | Imaging of live specimens in 35 mm dishes, Lab-Tek chambered coverslips and Ibidi uSlides using Oko-Lab cage type incubation chamber. DIC. Multi-point and stitching. FRAP. |
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