Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

...

Microscope Name

Upright or Inverted?

Objective Lenses

Laser Lines

Techniques

Radiance

Upright for slides

Up to 40x oil (water dipping lenses available)

458, 488, 514, 543, 635

Bio-Rad Microscience Radiance 2100 with Nikon 800 stand and three high-sensitivity GaAsP detectors for 3D imaging of fixed specimens on slides. DIC. Live imaging of specimens in gasket sealed chambers or open dishes possible using a heated stage adapter and lens heater. Fluorescence recovery after photobleaching (FRAP).

SPE

Upright for slides

Up to 63x oil (water dipping lenses available)

405, 488, 532, 640

Leica TCS SPE with DM2500 stand for 3D imaging of fixed specimens on slides. Live imaging of specimens in dishes possible with water dipping lenses using Oko-lab stage adapter or Warner Instruments perfusion chamber. DIC (not dipping lenses). Spectral imaging.

SPE

Upright for slides

Up to 63x oil (water dipping lenses available)

405, 488, 532, 640

Leica TCS SPE with DM2500 stand for 3D imaging of fixed specimens on slides. Live imaging of specimens in dishes possible with water dipping lenses using Oko-lab stage adapter or Warner Instruments perfusion chamber. DIC (not dipping lenses). Spectral imaging.

SPE3

Inverted

10x air to 63x oil

405, 488, 561, 640

Leica TCS SPE version 2 with DMI4000 stand for 3D imaging of specimens on slides and in dishes. DIC. Spectral Imaging. Motorised stage for multi-position acquisition and tiling. Matrix Screener software for high content screening.

SP5

Inverted

10x air to 63x oil (63x glycerol available)

405, 457, 476, 488, 495, 561, 594, 633

Leica TCS SP5 with DMI6000B for 3D imaging of specimens on slides and live imaging of specimens in 35 mm dishes and Lab-Tek chambered coverslips using Pe-Con incubation equipment. Spectral imaging. DIC. Motorised stage for multi-position acquisition and tiling. FRAP wizard. Förster Resonance Energy Transfer with FRET wizard. Experiment design using Live Data Mode. Matrix Screener for high content screening.

Spinning Disc Confocal Microscopes

Microscope Name

Upright or Inverted?

Objective Lenses

Laser Lines

Techniques

Vox

Inverted

10x air to 100x oil (60x water immersion available)

405, 488, 561, 640

Imaging of live specimens in 35 mm dishes, Lab-Tek chambered coverslips and Ibidi uSlides using Solent Scientific cage type incubation chamber. DIC. Multi-point and stitching. FRAP.

3iInverted10x air to 100x oil (40x water immersion available)405, 440, 488, 514, 561, 640Imaging of live specimens in 35 mm dishes, Lab-Tek chambered coverslips and Ibidi uSlides using Oko-Lab cage type incubation chamber. DIC. Multi-point and stitching. FRAP.

...