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Microscope Name | Upright or Inverted? | Objective Lenses | Laser Lines | Techniques |
|---|---|---|---|---|
Radiance | Upright for slides | Up to 40x oil (water dipping lenses available) | 458, 488, 514, 543, 635 | Bio-Rad Microscience Radiance 2100 with Nikon 800 stand and three high-sensitivity GaAsP detectors for 3D imaging of fixed specimens on slides. DIC. Live imaging of specimens in gasket sealed chambers or open dishes possible using a heated stage adapter and lens heater. Fluorescence recovery after photobleaching (FRAP). |
SPE | Upright for slides | Up to 63x oil (water dipping lenses available) | 405, 488, 532, 640 | Leica TCS SPE with DM2500 stand for 3D imaging of fixed specimens on slides. Live imaging of specimens in dishes possible with water dipping lenses using Oko-lab stage adapter or Warner Instruments perfusion chamber. DIC (not dipping lenses). Spectral imaging. |
SPE | Upright for slides | Up to 63x oil (water dipping lenses available) | 405, 488, 532, 640 | Leica TCS SPE with DM2500 stand for 3D imaging of fixed specimens on slides. Live imaging of specimens in dishes possible with water dipping lenses using Oko-lab stage adapter or Warner Instruments perfusion chamber. DIC (not dipping lenses). Spectral imaging. |
SPE3 | Inverted | 10x air to 63x oil | 405, 488, 561, 640 | Leica TCS SPE version 2 with DMI4000 stand for 3D imaging of specimens on slides and in dishes. DIC. Spectral Imaging. Motorised stage for multi-position acquisition and tiling. Matrix Screener software for high content screening. |
SP5 | Inverted | 10x air to 63x oil (63x glycerol available) | 405, 457, 476, 488, 495, 561, 594, 633 | Leica TCS SP5 with DMI6000B for 3D imaging of specimens on slides and live imaging of specimens in 35 mm dishes and Lab-Tek chambered coverslips using Pe-Con incubation equipment. Spectral imaging. DIC. Motorised stage for multi-position acquisition and tiling. FRAP wizard. Förster Resonance Energy Transfer with FRET wizard. Experiment design using Live Data Mode. Matrix Screener for high content screening. |
| Olympus FV1200 | Inverted | 10x to 60x oil. Silicon oil lenses | 405, 458, 488, 515, 559, 635 | Olympus FV1200 with IX83 microscope stand. Live imaging using Tokai-Hit stage adapter for dishes and chambered coverslips. Additional SIM scanner with 405 pulsed laser for ablation experiments and 440 nm laser for excitation of CFP. |
Spinning Disc Confocal Microscopes
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