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Microscope Name | Upright or Inverted? | Objective Lenses | Laser Lines | 37°C Incubation | Techniques |
|---|---|---|---|---|---|
Radiance | Upright | Suitable for coverslips and water dipping | 458, 488, 514, 543, 635 | Possible | Bio-Rad Microscience Radiance 2100 with Nikon 800 stand and three high-sensitivity GaAsP detectors for 3D imaging of fixed specimens on slides. DIC. Live imaging of specimens in gasket sealed chambers or open dishes possible using a heated stage adapter and lens heater. Fluorescence recovery after photobleaching (FRAP). |
SPE | Upright | Suitable for coverslips and water dipping | 405, 488, 532, 640 | Possible | Leica TCS SPE with DM2500 stand for 3D imaging of fixed specimens on slides. Live imaging of specimens in dishes possible with water dipping lenses using Oko-lab stage adapter or Warner Instruments perfusion chamber. DIC (not dipping lenses). Spectral imaging. |
SPE2 | Upright | Suitable for coverslips | 405, 488, 532, 640 | Possible | Leica TCS SPE with DM2500 stand for 3D imaging of fixed specimens on slides. Live imaging of specimens in dishes possible with water dipping lenses using Oko-lab stage adapter or Warner Instruments perfusion chamber. DIC (not dipping lenses). Spectral imaging. |
SPE3 | Inverted | Suitable for coverslips | 405, 488, 561, 640 | No | Leica TCS SPE version 2 with DMI4000 stand for 3D imaging of specimens on slides and in dishes. DIC. Spectral Imaging. Motorised stage for multi-position acquisition and tiling. Matrix Screener software for high content screening. |
SP5 | Inverted | Suitable for coverslips | 405, 457, 476, 488, 495, 561, 594, 633 | Yes | Leica TCS SP5 with DMI6000B for 3D imaging of specimens on slides and live imaging of specimens in 35 mm dishes and Lab-Tek chambered coverslips using Pe-Con incubation equipment. Spectral imaging. DIC. Motorised stage for multi-position acquisition and tiling. FRAP wizard. Förster Resonance Energy Transfer with FRET wizard. Experiment design using Live Data Mode. Matrix Screener for high content screening. |
| Olympus FV1200 | Inverted | Suitable for coverslips. Silicon lenses for live imaging | 405, 458, 488, 515, 559, 635 | Yes | Olympus FV1200 with IX83 microscope stand. Live imaging using Tokai-Hit stage adapter for dishes and chambered coverslips. Two spectral detectors, one PMT and two GaAsP detectors for high sensitivity imaging. DIC. Motorised stage. 32x and 60x silicon lenses for thick living specimens. Additional SIM scanner with 405 pulsed laser for ablation experiments and 440 nm laser for excitation of CFP. FRAP possible with or without SIM scanner. |
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