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Microscope Name | Upright or Inverted? | Fluorescence Colours | Key Features | Techniques |
|---|---|---|---|---|
Axioplan | Upright | Blue , Green , Red , Far-Red |
| Zeiss Axioplan 2 microscope with QImaging QClick camera for high sensitivity and high resolution fluorescence microscopy and differential interference contrast (DIC) of fixed specimens on slides |
Axio Imager | Upright | Blue , Green , Red , Far-Red . Cyan and Yellow available on request |
| Zeiss Axio Imager A1 microscope with QImaging Retiga EXi for high sensitivity and high resolution fluorescence microscopy and phase contrast of fixed specimens on slides |
Axioskop 3 | Upright | Blue , Green , Red |
| Zeiss Axioskop microscope with QImaging QICAM colour camera for moderate to high resolution fluorescence microscopy and imaging of histologically or histochemically stained specimens on slides. Motorised stage supports tiling and stitching of specimens. |
Inverted | Blue , Green , Red |
| Leica DMIRB microscope with Hamamatsu Orca NRB camera. Good for moderate resolution fluorescence microscopy and phase contrast of fixed/live specimens on slides, or in dishes and plates with glass or plastic bottoms | |
Leica 2 | Inverted | Blue , Cyan , Green , Red |
| Leica DMIRB microscope with Leica DFC7000T colour camera for high resolution fluorescence microscopy and colour imaging of histologically or histochemically stained specimens on slides, or in dishes and plates with glass or plastic bottoms |
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Use a confocal microscope if your specimen in thick, has significant out-of-focus blur, or if you need to process, analyse or visualise it in 3D.
Microscope Name | Upright or Inverted? |
|---|
Objective Lenses
Laser Lines |
|---|
| Features | Recommended Techniques |
|---|---|
Radiance | Upright |
Suitable for coverslips and water dipping
458, 488, 514, 543, 635 |
|
|
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| 3D imaging of fixed specimens |
SPE | Upright |
405, 488, 532, 640 |
Leica TCS SPE with DM2500 stand for 3D imaging of fixed specimens on slides. Live imaging of specimens in dishes possible with water dipping lenses using Oko-lab stage adapter or Warner Instruments perfusion chamber. DIC (not dipping lenses). Spectral imaging. | |
SPE2 | Upright |
405, 488, 532, 640 |
Leica TCS SPE with DM2500 stand for 3D imaging of fixed specimens on slides. Live imaging of specimens in dishes possible with water dipping lenses using Oko-lab stage adapter or Warner Instruments perfusion chamber. DIC (not dipping lenses). Spectral imaging. | ||
SPE3 | Inverted |
Suitable for coverslips
405, 488, 561, 640 |
Leica TCS SPE version 2 with DMI4000 stand for 3D imaging of specimens on slides and in dishes. DIC. Spectral Imaging. Motorised stage for multi-position acquisition and tiling. Matrix Screener software for high content screening. | ||
SP5 | Inverted |
Suitable for coverslips
405, 457, 476, 488, 495, 561, 594, 633 |
Leica TCS SP5 with DMI6000B for 3D imaging of specimens on slides and live imaging of specimens in 35 mm dishes and Lab-Tek chambered coverslips using Pe-Con incubation equipment. Spectral imaging. DIC. Motorised stage for multi-position acquisition and tiling. FRAP wizard. Förster Resonance Energy Transfer with FRET wizard. Experiment design using Live Data Mode. Matrix Screener for high content screening. | |
| Olympus FV1200 | Inverted |
| 405, 458, 488, 515, 559, 635 |
| Olympus FV1200 with IX83 microscope stand. Live imaging using Tokai-Hit stage adapter for dishes and chambered coverslips. Two spectral detectors, one PMT and two GaAsP detectors for high sensitivity imaging. DIC. Motorised stage. 32x and 60x silicon lenses for thick living specimens. Additional SIM scanner with 405 pulsed laser for ablation experiments and 440 nm laser for excitation of CFP. FRAP possible with or without SIM scanner. |
Spinning Disc Confocal Microscopes
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