Page Comparison

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Microscope Name	Upright or Inverted?	Laser Lines	37°C Incubation	Features	Recommended Techniques
Radiance	Upright	458, 488, 514, 543, 635	Possible	Bio-Rad Microscience Radiance 2100 with Nikon 800 stand Three high sensitivity GaAsP detectors Differential Interference Contrast Basic FRAP capability Incubation possible with stage and objective lens heaters	3D imaging of fixed specimens on slides. Other microscopes are recommended for live imaging of mammalian specimens and for more sophisticated FRAP capability.
SPE	Upright	405, 488, 532, 640	Possible	Leica TCS SPE with DM2500 stand Differential Interference Contrast Spectral imaging system One PMT detector Z Galvo high speed focus Water dipping lenses available Incubation possible with Warner Instruments perfusion chamber	3D imaging of fixed specimens on slides. Orthogonal scans (in XZ) using the Z Galvo. Live imaging of perfused specimens if an upright configuration is needed.
SPE2	Upright	405, 488, 532, 640	Possible	Leica TCS SPE with DM2500 stand Differential Interference Contrast Spectral imaging system One PMT detector Z Galvo high speed focus Water dipping lenses available Incubation possible with Oko-Lab stage adapter	3D imaging of fixed specimens on slides. Orthogonal scans (in XZ) using the Z Galvo. Live imaging of specimens in dishes possible with water dipping lenses using Oko-lab stage adapter if an upright configuration is needed.
SPE3	Inverted	405, 488, 561, 640	No	Leica TCS SPE version 2 with DMI4000 stand 561 nm laser instead of 532 nm laser Motorised stage Spectral imaging system One PMT detector Z Galvo high speed focus Differential Interference Contrast Matrix Screener software module	3D imaging of specimens on slides and in dishes with multi-position acquisition and tiling. 561 nm laser is closer to the excitation peak of most popular red fluorophores. Matrix Screener software for high content screening.
SP5	Inverted	405, 457, 476, 488, 495, 561, 594, 633	Yes	Leica TCS SP5 with DMI6000B stand Motorised stage Differential Interference Contrast Spectral imaging system Three high sensitivity Hybrid detectors One PMT detector FRAP and FRET Wizards Live Data Mode and Matrix Screener modules Pe-Con incubation equipment	3D imaging of specimens on slides and live imaging of specimens in 35 mm dishes and Lab-Tek chambered coverslips using Pe-Con incubation equipment. Multi-position acquisition and tiling. FRAP wizard. Förster Resonance Energy Transfer by Acceptor Photobleaching or Sensitised Emission using FRET wizards. Advanced experiment design using Live Data Mode. Matrix Screener for high content screening.
Olympus FV1200	Inverted	405, 458, 488, 515, 559, 635	Yes	Olympus FV1200 with IX83 microscope stand Motorised stage Differential Interference Contrast Spectral imaging for two PMT detectors Third non-spectral PMT with fluorescence filters Two high sensitivity GaAsP detectors Simultaneous (SIM) scanner with pulsed 405 nm laser and 440 continuous wave laser Ablation and FRAP capability 32x and 60x silicon lenses available Olympus FV1200 with IX83 microscope stand. Live imaging using Tokai-Hit stage adapter for Tokai-Hit incubation equipment	3D scanning of fixed specimens. Live imaging of specimens in 35 mm dishes and chambered coverslips. Two spectral detectors, one PMT and two GaAsP detectors for high sensitivity imaging. DIC. Motorised stage. 32x and 60x silicon lenses for thick living specimens. Additional SIM scanner with 405 pulsed laser for ablation experiments and 440 nm laser for excitation of CFP. FRAP possible with or without SIM scannerHigh sensitivity live imaging of green and red fluorophores using the GaAsP detectors. Imaging of thick living specimens using the silicon lenses to correct for the refractive index of cytoplasm. Ablation of cytoskeletal cortex in adherent cells using the pulsed 405 nm laser.

Spinning Disc Confocal Microscopes

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Old Version 107

New Version 108

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Spinning Disc Confocal Microscopes