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Microscope Name | Upright or Inverted? | Laser Lines | 37°C Incubation | Features | Recommended Techniques |
---|---|---|---|---|---|
Radiance | Upright | 458, 488, 514, 543, 635 | Possible |
| 3D imaging of fixed specimens on slides. Other microscopes are recommended for live imaging of mammalian specimens and for more sophisticated FRAP capability. |
SPE | Upright | 405, 488, 532, 640 | Possible |
| 3D imaging of fixed specimens on slides. Orthogonal scans (in XZ) using the Z Galvo. Live imaging of perfused specimens if an upright configuration is needed. |
SPE2 | Upright | 405, 488, 532, 640 | Possible |
| 3D imaging of fixed specimens on slides. Orthogonal scans (in XZ) using the Z Galvo. Live imaging of specimens in dishes possible with water dipping lenses using Oko-lab stage adapter if an upright configuration is needed. |
SPE3 | Inverted | 405, 488, 561, 640 | No |
| 3D imaging of specimens on slides and in dishes with multi-position acquisition and tiling. 561 nm laser is closer to the excitation peak of most popular red fluorophores. Matrix Screener software for high content screening. |
SP5 | Inverted | 405, 457, 476, 488, 495, 561, 594, 633 | Yes |
| 3D imaging of specimens on slides and live imaging of specimens in 35 mm dishes and Lab-Tek chambered coverslips using Pe-Con incubation equipment. Multi-position acquisition and tiling. FRAP wizard. Förster Resonance Energy Transfer by Acceptor Photobleaching or Sensitised Emission using FRET wizards. Advanced experiment design using Live Data Mode. Matrix Screener for high content screening. |
Olympus FV1200 | Inverted | 405, 458, 488, 515, 559, 635 | Yes |
| 3D scanning of fixed specimens. Live imaging of specimens in 35 mm dishes and chambered coverslips. Two spectral detectors, one PMT and two GaAsP detectors for high sensitivity imaging. DIC. Motorised stage. 32x and 60x silicon lenses for thick living specimens. Additional SIM scanner with 405 pulsed laser for ablation experiments and 440 nm laser for excitation of CFP. FRAP possible with or without SIM scannerHigh sensitivity live imaging of green and red fluorophores using the GaAsP detectors. Imaging of thick living specimens using the silicon lenses to correct for the refractive index of cytoplasm. Ablation of cytoskeletal cortex in adherent cells using the pulsed 405 nm laser. |
Spinning Disc Confocal Microscopes
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