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Microscope Name | Upright or Inverted? | Laser Lines | Key Features | Techniques |
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SPE | Upright | 405, 488, 532, 640 |
| Leica TCS SPE with DM2500 stand, a single PMT fluorescence detector and DIC. Most suitable for 3D imaging of fixed specimens on slides. Samples can be scanned orthogonally (in XZ) using the Z Galvo. Live imaging is possible using a Warner Instruments perfusion chamber and dipping lenses. |
SPE2 | Upright | 405, 488, 532, 640 |
| Leica TCS SPE with DM2500 stand, single PMT fluorescence detector and DIC. Most suitable for 3D imaging of fixed specimens on slides. Samples can be scanned orthogonally (in XZ) using the Z Galvo. Live imaging is possible using an Oko-Lab stage-top incubator and dipping lenses. |
SPE3 | Inverted | 405, 488, 561, 640 |
| Leica TCS SPE with DMI4000 stand, single PMT fluorescence detector and DIC. Most suitable for 3D imaging of fixed specimens on slides and in dishes with multi-position acquisition and tiling. at multiple positions or with tiling. Suitable for scanning gridded dishes for CLEM. In comparison to other SPE confocal microscopes the 561 nm laser is closer to the excitation peak of most popular red fluorophores. Fluorescence and DIC on gridded 35 mm dishes for correlative light and electron microscopy. Matrix Screener software is available for high content screening type applications. |
SP5 | Inverted | 405, 457, 476, 488, 495, 561, 594, 633 |
| 3D imaging of specimens on slides and live imaging of specimens in 35 mm dishes and Lab-Tek chambered coverslips using Pe-Con incubation equipment. Multi-position acquisition and tiling. Fluorescence recovery after photobleaching (FRAP). Förster Resonance Energy Transfer by Acceptor Photobleaching or Sensitised Emission using FRET wizards. Fluorescence and DIC on gridded 35 mm dishes for correlative light and electron microscopy. Advanced experiment design using Live Data Mode (e.g. reconfiguring acquisition for SRRF). Matrix Screener for high content screening, export of data as OME TIFF. |
Olympus FV1200 | Inverted | 405, 458, 488, 515, 559, 635 Sim Scanner: 405 (pulsed), 440 |
| 3D scanning of fixed specimens. Live imaging of specimens in 35 mm dishes and chambered coverslips. Highly sensitivite live imaging of green and red fluorophores using the GaAsP detectors. Imaging of thick living specimens using the silicon lenses to correct for the refractive index of cytoplasm. Tiling of large specimens using the motorised stage. Ablation of cytoskeletal cortex in adherent cells using the pulsed 405 nm laser. Fluorescence and DIC on gridded 35 mm dishes for correlative light and electron microscopy. |
Radiance | Upright | 458, 488, 514, 543, 635 |
| 3D imaging of fixed specimens on slides. Other microscopes are recommended for live imaging of mammalian specimens and for more sophisticated FRAP capability. |
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