Microscope Name | Upright or Inverted? | Laser Lines | Live Imaging | Key Features | Techniques |
|---|
SPE | Upright | 405, 488, 532, 640 | Perfusion chamber and dipping lenses on request | - Leica TCS SPE with DM2500 stand
- Differential Interference Contrast
- Spectral imaging system
- One PMT detector
- Z Galvo high speed focus
- Water dipping lenses available
- Incubation possible with Warner Instruments perfusion chamber
| - Leica TCS SPE with DM2500 stand. One PMT detector.
- Transmitted light contrast by DIC.
- Most suitable for 3D imaging of fixed specimens on slides.
- Live imaging is possible but image acquisition is not fast.
- Samples can be scanned orthogonally (in XZ) using the Z Galvo.
|
SPE2 | Upright | 405, 488, 532, 640 | Oko-Lab stage adapter and dipping lenses on request | - Leica TCS SPE with DM2500 stand
- Differential Interference Contrast
- Spectral imaging system
- One PMT detector
- Z Galvo high speed focus
- Water dipping lenses available
- Incubation possible with Oko-Lab stage adapter
| - Leica TCS SPE with DM2500 stand. One PMT detector.
- Transmitted light contrast by DIC.
- Most suitable for 3D imaging of fixed specimens on slides.
- Live imaging is possible but image acquisition is not fast.
- Samples can be scanned orthogonally (in XZ) using the Z Galvo.
|
SPE3 | Inverted | 405, 488, 561, 640 | No incubation equipment is available for this microscope | - Leica TCS SPE version 2 with DMI4000 stand
- 561 nm laser instead of 532 nm laser
- Motorised stage
- Spectral imaging system
- One PMT detector
- Z Galvo high speed focus
- Differential Interference Contrast
- Matrix Screener software module
| - Leica TCS SPE with DMI4000 stand. One PMT detector. DIC.
- Most suitable for imaging of fixed specimens in slides and dishes.
- Inverted stand suitable for fluorescence and DIC on grids for CLEM.
- Motorised stage for multi-position imaging and tiling.
- 561 nm laser is close to the excitation peak of popular red fluorophores.
- Matrix Screener module for high content screening applications.
|
SP5 | Inverted | 405, 457, 476, 488, 495, 561, 594, 633 | Pe-Con stage-top incubator for 35 mm dishes | - Leica TCS SP5 with DMI6000B stand
- Motorised stage
- Differential Interference Contrast
- Spectral imaging system
- Three high sensitivity Hybrid detectors and one PMT
- FRAP and FRET Wizards
- Live Data Mode and Matrix Screener modules
- Pe-Con incubation equipment
| - Leica TCS SP5 with DMI6000B stand. Three
- 3x sensitive Hybrid detectors, one 1x PMT
- 4D (3D+T) imaging of live and fixed specimens
- Inverted stand suitable for fluorescence and DIC on grids for CLEM
- Motorised stage for multi-position acquisition and tiling
- Multi-well plate insert available
- FRAP and FRET Acceptor Photobleaching or Sensitised Emission.
- Live Data Mode and Matrix Screener for complex experiment design.
|
| Olympus FV1200 | Inverted | 405, 458, 488, 515, 559, 635 Sim Scanner: 405 (pulsed), 440 | Tokai-Hit stage-top incubator with adaptors for dishes and chambered coverslips | - Olympus FV1200 with IX83 microscope stand
- Motorised stage
- Differential Interference Contrast
- Spectral imaging for two PMT detectors
- Third non-spectral PMT with fluorescence filters
- Two high sensitivity GaAsP detectors
- Simultaneous (Sim) scanner with pulsed 405 nm laser and 440 continuous wave laser
- Ablation and FRAP capability
- 32x and 60x silicon lenses available
- Tokai-Hit incubation equipment
| - Olympus FV1200 with IX83 microscope stand
- 4D (3D+T) imaging of live and fixed specimens
- 3x PMTs and 2x sensitive GaAsP detectors (for green and red dyes)
- Second scanner for simultaneous imaging and ablation/FRAP
- Inverted stand suitable for fluorescence and DIC on grids for CLEM
- Motorised stage for multi-position acquisition and tiling
- 3D scanning of fixed specimens. Live imaging of specimens in 35 mm dishes and chambered coverslips. Highly sensitivite live imaging of green and red fluorophores using the GaAsP detectors. Imaging of thick living specimens using the silicon lenses to correct for the refractive index of cytoplasm. Tiling of large specimens using the motorised stage. Ablation of cytoskeletal cortex in adherent cells using the pulsed 405 nm laser. Fluorescence and DIC on gridded 35 mm dishes for correlative light and electron microscopy.Silicon lenses for imaging thick specimens
- Time Controller for complex experiment design
|
Radiance | Upright | 458, 488, 514, 543, 635 |
| - Bio-Rad Microscience Radiance 2100 with Nikon 800 stand
- Three high sensitivity GaAsP detectors
- Differential Interference Contrast
- Basic FRAP capability
- Incubation possible with stage and objective lens heaters
| 3D imaging of fixed specimens on slides. Other microscopes are recommended for live imaging of mammalian specimens and for more sophisticated FRAP capability. |