Microscope Name | Upright or Inverted? | Laser Lines | Live Imaging | Key Features | Techniques |
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SPE | Upright | 405, 488, 532, 640 | Perfusion chamber and dipping lenses on request | - Leica TCS SPE with DM2500 stand
- Differential Interference Contrast
- Spectral imaging system
- One PMT detector
- Z Galvo high speed focus
- Water dipping lenses available
- Incubation possible with Warner Instruments perfusion chamber
| - Leica TCS SPE with DM2500 stand. One PMT detector
- Transmitted light contrast by DIC
- Most suitable for 3D imaging of fixed specimens on slides.
- Transmitted light contrast by DIC
- Live imaging is possible but image acquisition is not fast.
- Samples can be scanned orthogonally (in XZ) using the Z Galvo.
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SPE2 | Upright | 405, 488, 532, 640 | Oko-Lab stage adapter and dipping lenses on request | - Leica TCS SPE with DM2500 stand
- Differential Interference Contrast
- Spectral imaging system
- One PMT detector
- Z Galvo high speed focus
- Water dipping lenses available
- Incubation possible with Oko-Lab stage adapter
| - Leica TCS SPE with DM2500 stand. One PMT detectorTransmitted light contrast by DIC
- Most suitable for 3D imaging of fixed specimens on slides.
- Transmitted light contrast by DIC
- Live imaging is possible but image acquisition is not fast.
- Samples can be scanned orthogonally (in XZ) using the Z Galvo.
|
SPE3 | Inverted | 405, 488, 561, 640 | No incubation equipment is available for this microscope | - Leica TCS SPE version 2 with DMI4000 stand
- 561 nm laser instead of 532 nm laser
- Motorised stage
- Spectral imaging system
- One PMT detector
- Z Galvo high speed focus
- Differential Interference Contrast
- Matrix Screener software module
| - Leica TCS SPE with DMI4000 stand. One PMT detector
- Most suitable for imaging of fixed specimens in slides and dishes.
- Inverted stand suitable for fluorescence and DIC on grids for CLEM.
- Motorised stage for multi-position imaging and tiling.
- 561 nm laser is close to the excitation peak of popular red fluorophores.
- Matrix Screener module for high content screening applications.
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SP5 | Inverted | 405, 457, 476, 488, 495, 561, 594, 633 | Pe-Con stage-top incubator for 35 mm dishes | - Leica TCS SP5 with DMI6000B stand
- Motorised stage
- Differential Interference Contrast
- Spectral imaging system
- Three high sensitivity Hybrid detectors and one PMT
- FRAP and FRET Wizards
- Live Data Mode and Matrix Screener modules
- Pe-Con incubation equipment
| - Leica TCS SP5 with DMI6000B stand
- 3x sensitive Hybrid detectors, 1x PMT
- 4D (3D+T) imaging of live and fixed specimens
- Inverted stand suitable for fluorescence and DIC on grids for CLEM
- Motorised stage for multi-position acquisition and tiling
- FRAP and FRET Acceptor Photobleaching or Sensitised Emission.
- Live Data Mode and Matrix Screener for complex experiment design.
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| Olympus FV1200 | Inverted | 405, 458, 488, 515, 559, 635 Sim Scanner: 405 (pulsed), 440 | Tokai-Hit stage-top incubator with adaptors for dishes and chambered coverslips | - Olympus FV1200 with IX83 microscope stand
- Motorised stage
- Differential Interference Contrast
- Spectral imaging for two PMT detectors
- Third non-spectral PMT with fluorescence filters
- Two high sensitivity GaAsP detectors
- Simultaneous (Sim) scanner with pulsed 405 nm laser and 440 continuous wave laser
- Ablation and FRAP capability
- 32x and 60x silicon lenses available
- Tokai-Hit incubation equipment
| - Olympus FV1200 with IX83 microscope stand
- 4D (3D+T) imaging of live and fixed specimens
- 3x PMTs and 2x sensitive GaAsP detectors (for green and red dyes)
- Second scanner for simultaneous imaging and ablation/FRAP
- Inverted stand suitable for fluorescence and DIC on grids for CLEM
- Motorised stage for multi-position acquisition and tiling
- Silicon lenses for imaging thick specimens
- Time Controller for complex experiment design
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Radiance | Upright | 458, 488, 514, 543, 635 | Tokai-Hit stage heater and Bioptechs objective lens heater available on request | - Bio-Rad Microscience Radiance 2100 with Nikon 800 stand
- Three high sensitivity GaAsP detectors
- Differential Interference Contrast
- Basic FRAP capability
- Incubation possible with stage and objective lens heaters
| - Bio-Rad Radiance 2100 with Nikon E800 microscope stand
- 3D imaging of fixed specimens on slides
. Other microscopes are recommended for live imaging of mammalian specimens and for more sophisticated FRAP capability.- Transmitted light contrast by DIC
- 3x high sensitivity GaAsP detectors
- Basic FRAP capability
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