Microscope Name | Upright or Inverted? | Laser Lines | Live Imaging | Key Features | Techniques |
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SPE | Upright | 405, 488, 532, 640 | Perfusion chamber and dipping lenses on request | - Leica TCS SPE with DM2500 stand
| Differential Interference ContrastSpectral imaging systemOne PMT detectorZ Galvo high speed focusWater dipping lenses availableIncubation possible with Warner Instruments perfusion chamber- Leica TCS SPE with DM2500 stand. One PMT detector
- Most suitable for 3D imaging of fixed specimens on slides.
- Transmitted light contrast by DIC
- Live imaging is possible but image acquisition is not fast.
- Samples can be scanned orthogonally (in XZ) using the Z Galvo.
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SPE2 | Upright | 405, 488, 532, 640 | Oko-Lab stage adapter and dipping lenses on request | - Leica TCS SPE with DM2500 stand
| Differential Interference ContrastSpectral imaging systemOne PMT detectorZ Galvo high speed focusWater dipping lenses availableIncubation possible with Oko-Lab stage adapter- Leica TCS SPE with DM2500 stand. One PMT detector
- Most suitable for 3D imaging of fixed specimens on slides.
- Transmitted light contrast by DIC
- Live imaging is possible but image acquisition is not fast.
- Samples can be scanned orthogonally (in XZ) using the Z Galvo.
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SPE3 | Inverted | 405, 488, 561, 640 | No incubation equipment is available for this microscopeLeica TCS SPE version 2 with DMI4000 stand561 nm laser instead of 532 nm laserMotorised stageSpectral imaging systemOne PMT detectorZ Galvo high speed focusDifferential Interference ContrastMatrix Screener software module | - Leica TCS SPE with DMI4000 stand. One PMT detector
- Most suitable for imaging of fixed specimens in slides and dishes.
- Inverted stand suitable for fluorescence and DIC on grids for CLEM.
- Motorised stage for multi-position imaging and tiling.
- 561 nm laser is close to the excitation peak of popular red fluorophores.
- Matrix Screener module for high content screening applications.
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SP5 | Inverted | 405, 457, 476, 488, 495, 561, 594, 633 | Pe-Con stage-top incubator for 35 mm dishesLeica TCS SP5 with DMI6000B standMotorised stageDifferential Interference ContrastSpectral imaging systemThree high sensitivity Hybrid detectors and one PMTFRAP and FRET WizardsLive Data Mode and Matrix Screener modulesPe-Con incubation equipment | - Leica TCS SP5 with DMI6000B stand
- 3x sensitive Hybrid detectors, 1x PMT
- 4D (3D+T) imaging of live and fixed specimens
- Inverted stand suitable for fluorescence and DIC on grids for CLEM
- Motorised stage for multi-position acquisition and tiling
- FRAP and FRET Acceptor Photobleaching or Sensitised Emission.
- Live Data Mode and Matrix Screener for complex experiment design.
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| Olympus FV1200 | Inverted | 405, 458, 488, 515, 559, 635 Sim Scanner: 405 (pulsed), 440 | Tokai-Hit stage-top incubator with adaptors for dishes and chambered coverslips | - Olympus FV1200 with IX83 microscope stand
- Motorised stage
- Differential Interference Contrast
- Spectral imaging for two PMT detectors
- Third non-spectral PMT with fluorescence filters
- Two high sensitivity GaAsP detectors
- Simultaneous (Sim) scanner with pulsed 405 nm laser and 440 continuous wave laser
- Ablation and FRAP capability
- 32x and 60x silicon lenses available
- Tokai-Hit incubation equipment
- Olympus FV1200 with IX83 microscope stand
- 4D (3D+T) imaging of live and fixed specimens
- 3x PMTs and 2x sensitive GaAsP detectors (for green and red dyes)
- Second scanner for simultaneous imaging and ablation/FRAP
- Inverted stand suitable for fluorescence and DIC on grids for CLEM
- Motorised stage for multi-position acquisition and tiling
- Silicon lenses for imaging thick specimens
- Time Controller for complex experiment design
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Radiance | Upright | 458, 488, 514, 543, 635 | Tokai-Hit stage heater and Bioptechs objective lens heater available on requestBio-Rad Microscience Radiance 2100 with Nikon 800 standThree high sensitivity GaAsP detectorsDifferential Interference ContrastBasic FRAP capabilityIncubation possible with stage and objective lens heaters | - Bio-Rad Radiance 2100 with Nikon E800 microscope stand
- 3D imaging of fixed specimens on slides
- Transmitted light contrast by DIC
- 3x high sensitivity GaAsP detectors
- Basic FRAP capability
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