...
Use a confocal microscope if your specimen in thick, has significant out-of-focus blur, or if you need to process, analyse or visualise it in 3D.
Microscope Name | Upright or Inverted? | Laser Lines | Features | Live Imaging | Techniques |
|---|
SPE | Upright | 405, 488, 532, 640 |
Perfusion chamber and dipping lenses on request | Leica TCS SPE with DM2500 stand. |
One | 1x PMT detector. Transmitted light detector. DIC. | Perfusion chamber and dipping lenses on request | Most suitable for 3D imaging of fixed specimens on slides. |
Transmitted light contrast by DIC Live imaging is possible but image acquisition is not fast. |
Samples can be scanned orthogonally (in XZ) using the Z Galvo. |
SPE2 | Upright | 405, 488, 532, 640 |
| Oko-Lab stage adapter and dipping lenses on request | - Leica TCS SPE with DM2500 stand. One PMT detector
- Most suitable for 3D imaging of fixed specimens on slides.
- Transmitted light contrast by DIC
- Live imaging is possible but image acquisition is not fast.
- Samples can be scanned orthogonally (in XZ) using the Z Galvo.
|
SPE3 | Inverted | 405, 488, 561, 640 |
| No incubation equipment is available for this microscope | - Leica TCS SPE with DMI4000 stand. One PMT detector
- Most suitable for imaging of fixed specimens in slides and dishes.
- Inverted stand suitable for fluorescence and DIC on grids for CLEM.
- Motorised stage for multi-position imaging and tiling.
- 561 nm laser is close to the excitation peak of popular red fluorophores.
- Matrix Screener module for high content screening applications.
|
SP5 | Inverted | 405, 457, 476, 488, 495, 561, 594, 633 |
| Pe-Con stage-top incubator for 35 mm dishes | - Leica TCS SP5 with DMI6000B stand
- 3x sensitive Hybrid detectors, 1x PMT
- 4D (3D+T) imaging of live and fixed specimens
- Inverted stand suitable for fluorescence and DIC on grids for CLEM
- Motorised stage for multi-position acquisition and tiling
- FRAP and FRET Acceptor Photobleaching or Sensitised Emission.
- Live Data Mode and Matrix Screener for complex experiment design.
|
| Olympus FV1200 | Inverted | 405, 458, 488, 515, 559, 635 Sim Scanner: 405 (pulsed), 440 |
| Tokai-Hit stage-top incubator with adaptors for dishes and chambered coverslips | - Olympus FV1200 with IX83 microscope stand
- 4D (3D+T) imaging of live and fixed specimens
- 3x PMTs and 2x sensitive GaAsP detectors (for green and red dyes)
- Second scanner for simultaneous imaging and ablation/FRAP
- Inverted stand suitable for fluorescence and DIC on grids for CLEM
- Motorised stage for multi-position acquisition and tiling
- Silicon lenses for imaging thick specimens
- Time Controller for complex experiment design
|
Radiance | Upright | 458, 488, 514, 543, 635 |
| Tokai-Hit stage heater and Bioptechs objective lens heater available on request | - Bio-Rad Radiance 2100 with Nikon E800 microscope stand
- 3D imaging of fixed specimens on slides
- Transmitted light contrast by DIC
- 3x high sensitivity GaAsP detectors
- Basic FRAP capability
|
Spinning Disc Confocal Microscopes
...