Microscope Name | Upright or Inverted? | Laser Lines | Features | Live Imaging | Techniques |
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SPE | Upright | 405, 488, 532, 640 | Leica TCS SPE with DM2500 stand. 1x PMT detector. Transmitted light detector. DIC. | Perfusion chamber and dipping lenses on request | Most suitable for 3D imaging of fixed specimens on slides. Live imaging is possible but image acquisition is not fast. Samples can be scanned orthogonally Orthogonal (in XZ) using scans possible with the Z Galvo. |
SPE2 | Upright | 405, 488, 532, 640 |
| Oko-Lab stage adapter and dipping lenses on request | - Leica TCS SPE with DM2500 stand. One PMT detector
- Most suitable for 3D imaging of fixed specimens on slides.
- Transmitted light contrast by DIC
- Live imaging is possible but image acquisition is not fast.
- Samples can be scanned orthogonally (in XZ) using the Z Galvo.
|
SPE3 | Inverted | 405, 488, 561, 640 |
| No incubation equipment is available for this microscope | - Leica TCS SPE with DMI4000 stand. One PMT detector
- Most suitable for imaging of fixed specimens in slides and dishes.
- Inverted stand suitable for fluorescence and DIC on grids for CLEM.
- Motorised stage for multi-position imaging and tiling.
- 561 nm laser is close to the excitation peak of popular red fluorophores.
- Matrix Screener module for high content screening applications.
|
SP5 | Inverted | 405, 457, 476, 488, 495, 561, 594, 633 |
| Pe-Con stage-top incubator for 35 mm dishes | - Leica TCS SP5 with DMI6000B stand
- 3x sensitive Hybrid detectors, 1x PMT
- 4D (3D+T) imaging of live and fixed specimens
- Inverted stand suitable for fluorescence and DIC on grids for CLEM
- Motorised stage for multi-position acquisition and tiling
- FRAP and FRET Acceptor Photobleaching or Sensitised Emission.
- Live Data Mode and Matrix Screener for complex experiment design.
|
Olympus FV1200 | Inverted | 405, 458, 488, 515, 559, 635 Sim Scanner: 405 (pulsed), 440 |
| Tokai-Hit stage-top incubator with adaptors for dishes and chambered coverslips | - Olympus FV1200 with IX83 microscope stand
- 4D (3D+T) imaging of live and fixed specimens
- 3x PMTs and 2x sensitive GaAsP detectors (for green and red dyes)
- Second scanner for simultaneous imaging and ablation/FRAP
- Inverted stand suitable for fluorescence and DIC on grids for CLEM
- Motorised stage for multi-position acquisition and tiling
- Silicon lenses for imaging thick specimens
- Time Controller for complex experiment design
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Radiance | Upright | 458, 488, 514, 543, 635 |
| Tokai-Hit stage heater and Bioptechs objective lens heater available on request | - Bio-Rad Radiance 2100 with Nikon E800 microscope stand
- 3D imaging of fixed specimens on slides
- Transmitted light contrast by DIC
- 3x high sensitivity GaAsP detectors
- Basic FRAP capability
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