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One Most suitable for

Microscope Name

Upright or Inverted?

Laser Lines

FeaturesLive Imaging

Techniques

SPE

Upright

405, 488, 532, 640

Leica TCS SPE with DM2500 stand. 1x PMT detector. DIC imaging.Perfusion chamber and dipping lenses on request.

3D imaging of fixed specimens on slides. Live imaging possible but not fast. Orthogonal (XZ) scans possible with the Z Galvo.

SPE2

Upright

405, 488, 532, 640

Leica TCS SPE with DM2500 stand. 1x PMT detector. DIC imaging.Oko-Lab stage adapter and dipping lenses on request.

3D imaging of fixed specimens on slides. Live imaging possible but not fast. Orthogonal (XZ) scans possible with the Z Galvo

SPE3

Inverted

405, 488, 561, 640

No incubation equipment is available for this microscopeLeica TCS SPE with DMI4000 stand. 1x PMT detector. DIC imaging. Motorised stage. Matrix screener module.No incubation equipment is available for this microscope

3D imaging of fixed specimens in slides and dishes.

Inverted stand suitable for fluorescence

Fluorescence and DIC on grids for CLEM.

Motorised stage for multi

Multi-position imaging and tiling.

  • 561 nm laser is close to the excitation peak of popular red fluorophores.

    • Matrix Screener

    module

    for high content screening applications.

    SP5

    Inverted

    405, 457, 476, 488, 495, 561, 594, 633


    		Pe-Con stage-top incubator for 35 mm dishes
    • Leica TCS SP5 with DMI6000B stand
    • 3x sensitive Hybrid detectors, 1x PMT
    • 4D (3D+T) imaging of live and fixed specimens
    • Inverted stand suitable for fluorescence and DIC on grids for CLEM
    • Motorised stage for multi-position acquisition and tiling
    • FRAP and FRET Acceptor Photobleaching or Sensitised Emission.
    • Live Data Mode and Matrix Screener for complex experiment design.
    Olympus FV1200Inverted

    405, 458, 488, 515, 559, 635

    Sim Scanner:

    405 (pulsed), 440


    		Tokai-Hit stage-top incubator with adaptors for dishes and chambered coverslips
    • Olympus FV1200 with IX83 microscope stand
    • 4D (3D+T) imaging of live and fixed specimens
    • 3x PMTs and 2x sensitive GaAsP detectors (for green and red dyes)
    • Second scanner for simultaneous imaging and ablation/FRAP
    • Inverted stand suitable for fluorescence and DIC on grids for CLEM
    • Motorised stage for multi-position acquisition and tiling
    • Silicon lenses for imaging thick specimens
    • Time Controller for complex experiment design

    Radiance

    Upright

    458, 488, 514, 543, 635


    		Tokai-Hit stage heater and Bioptechs objective lens heater available on request
    • Bio-Rad Radiance 2100 with Nikon E800 microscope stand
    • 3D imaging of fixed specimens on slides
    • Transmitted light contrast by DIC
    • 3x high sensitivity GaAsP detectors
    • Basic FRAP capability

    ...