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Microscope Name | Upright or Inverted? | Laser Lines | Features | Live Imaging | Techniques |
|---|---|---|---|---|---|
SPE | Upright | 405, 488, 532, 640 | Leica TCS SPE with DM2500 stand. 1x PMT detector. DIC imaging. | Perfusion chamber and dipping lenses on request. | 3D imaging of fixed specimens on slides. Live imaging possible but not fast. Orthogonal (XZ) scans possible with the Z Galvo. |
SPE2 | Upright | 405, 488, 532, 640 | Leica TCS SPE with DM2500 stand. 1x PMT detector. DIC imaging. | Oko-Lab stage adapter and dipping lenses on request. | 3D imaging of fixed specimens on slides. Live imaging possible but not fast. Orthogonal (XZ) scans possible with the Z Galvo. |
SPE3 | Inverted | 405, 488, 561, 640 | Leica TCS SPE with DMI4000 stand. 1x PMT detector. DIC imaging. Motorised stage. Matrix screener module. | No incubation equipment is available for this microscope. | 3D imaging of fixed specimens in slides and dishes. Fluorescence and DIC on grids for CLEM. Multi-position imaging and tiling. Matrix Screener for high content screening applications. |
SP5 | Inverted | 405, 457, 476, 488, 495, 561, 594, 633 | Leica TCS SP5 with DMI6000B stand. 3x Hybrid and 1x PMT decectors. DIC. Motorised stage. FRAP and FRET wizards. Live Data Mode and Matrix Screener. | Pe-Con stage-top incubator for 35 mm dishes. | 4D (3D+T) imaging of live and fixed specimens. CLEM. Multi-position acquisition and tiling. FRAP and FRET Acceptor Photobleaching or Sensitised Emission. Live Data Mode and Matrix Screener for complex experiment design. |
| Olympus FV1200 | Inverted | 405, 458, 488, 515, 559, 635 Sim Scanner: 405 (pulsed), 440 | Olympus FV1200 with IX83 stand. 3x PMT, 2x GaAsP detectors. Second scanner for simultaneous imaging and ablation. Motorised stage. Silicon lenses. Time Controller module. | Tokai-Hit stage-top incubator with adaptors for dishes and chambered coverslips. | 4D (3D+T) imaging of live and fixed specimens. High sensitivity imaging of green and red dyes with GaAsP detectors. Ablation of actin cortex. CLEM. Multi-position acquisition and tiling. Silicon lenses for imaging thick specimens. Time Controller for complex experiment design. |
Radiance | Upright | 458, 488, 514, 543, 635 | Bio-Rad radiance 2100 with Nikon E800 stand. 3x GaAsP detectors. Transmitted light contrast by DIC. Basic FRAP capability. | Tokai-Hit stage heater and Bioptechs objective lens heater available on request | 3D imaging of fixed specimens on slides . |
Spinning Disc Confocal Microscopes
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