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Use spinning disc microscopes for high-speed live confocal imaging of samples.

Microscope Name	Upright or Inverted?	Laser Lines	Key Features	Live Imaging	Techniques
Vox	Inverted	405, 488, 561, 640	Perkin Elmer Ultraview Vox

with Yokogawa CSU-X1

spinning disc

DSU and Nikon TiE

microscope

stand. Hamamatsu C9100-13 EMCCD camera

. Photokinesis Unit

(PKU)

for FRAP

. Motorised stage

Prior NanoscanZ piezo focus

Oko-Lab cage incubator

. Piezo focus.	Imaging of live specimens in 35 mm dishes, Lab-Tek chambered coverslips and Ibidi uSlides using Solent Scientific cage type incubation chamber.	Sensitive high-speed live confocal imaging. This machine has a smaller field of view than the 3i but the image acquisition speed is higher. DIC. Multi-point and stitching. Good for FRAP. CLEM.
3i	Inverted	405, 440, 488, 514, 561, 640	Yokogawa CSU-W1 spinning disc Zeiss Axio Observer Z1 microscope Photometrics Prime 95b camera Andor iXon 888 Life EMCCD camera Vector system for FRAP Motorised stage ASI high speed piezo focus Oko-Lab cage incubator		Imaging of live specimens in 35 mm dishes, Lab-Tek chambered coverslips and Ibidi uSlides using Oko-Lab cage type incubation chamber. DIC. Multi-point and stitching. FRAP.

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