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Microscope Name

Upright or Inverted?

Laser Lines

Key FeaturesLive Imaging

Techniques

Vox

Inverted

405, 488, 561, 640

Perkin Elmer Ultraview Vox with Yokogawa CSU-X1 DSU and Nikon TiE stand. Hamamatsu C9100-13 EMCCD camera. Photokinesis Unit for FRAP. Motorised stage. Piezo focus.

Imaging of live specimens in 35 mm dishes, Lab-Tek chambered coverslips and Ibidi uSlides using Solent Scientific cage type incubation chamber.

Sensitive and gentle high-speed live confocal imaging. This machine has a smaller field of view than the 3i but the image acquisition speed is higher. DIC. Multi-point and stitching. Good for FRAP. CLEM.

3iInverted405, 440, 488, 514, 561, 640

Yokogawa CSU-W1 spinning disc and Zeiss Axio Observer Z1 stand. Photometrics Prime 95b and Andor iXon 888 Life EMCCD cameras. Vector for FRAP. Motorised stage. High speed piezo focus

Oko-Lan Lab cage incubator with CO2 chamber and adaptors for 35 mm dishes, chambered coverslips and multi-well plates.Sensitive and gentle high-speed live confocal imaging. This machine has a larger field of view than the Vox but the image acquisition speed is not quite as high. Multi-point and stitching. FRAP.

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