...
Microscope Name | Upright or Inverted? | Laser Lines | Key Features | Live Imaging | Techniques |
|---|---|---|---|---|---|
Vox | Inverted | 405, 488, 561, 640 | Perkin Elmer Ultraview Vox with Yokogawa CSU-X1 DSU and Nikon TiE stand. Hamamatsu C9100-13 EMCCD camera. Photokinesis Unit for FRAP. Motorised stage. Piezo focus. | Imaging of live specimens in 35 mm dishes, Lab-Tek chambered coverslips and Ibidi uSlides using Solent Scientific cage type incubation chamber. | Sensitive and gentle high-speed live confocal imaging. This machine has a smaller field of view than the 3i but the image acquisition speed is higher. DIC. Multi-point and stitching. Good for FRAP. CLEM. |
| 3i | Inverted | 405, 440, 488, 514, 561, 640 | Yokogawa CSU-W1 spinning disc and Zeiss Axio Observer Z1 stand. Photometrics Prime 95b and Andor iXon 888 Life EMCCD cameras. Vector for FRAP. Motorised stage. High speed piezo focus | Oko-Lab cage incubator with CO2 chamber and adaptors for 35 mm dishes, chambered coverslips and multi-well plates. | Sensitive and gentle high-speed live confocal imaging. This machine has a larger field of view than the Vox but the image acquisition speed is not quite as high. Multi-point and stitching. FRAP. |
Time-lapse inverted microscope systems
Microscope |
|---|
| Upright or Inverted? | Fluorescence Colours |
|---|
Key Features | Live Imaging | Techniques |
|---|---|---|
GFP1 |
10x to 40x air and LWD (oil lenses available)
Green and Red
| Inverted | Green, Red, Far-Red | Zeiss Axio Observer Z1 stand with Hamamatsu Orca Flash4.0 v2 sCMOS camera. Motorised stage. Fluorescence. Phase Contrast. | Solent Scientific chamber with adaptor for multi-well plate. Other adaptors available on request. | Optimally configured for 'screening' of multiple positions in multi-well plates and other vessels at low to moderate resolution and high sensitivity using phase contrast and fluorescence. |
GFP2
10x to 40x air and LWD (oil lenses available)
GFP2 | Inverted | Cyan, Green, Red and Far-Red | Greyscale high sensitivity CCD | Optimally configured for 'screening' of multiple positions in multi-well plates and other vessels at low to moderate resolution and high sensitivity using phase contrast and fluorescence. Stage adapters support Nunc and Mat-Tek multi-well plates and 35 mm Mat-Tek dishes. If you need to use another vessel or adapter you must contact Andrew Vaughan |
N-STORM (coming soon)
GFP3 | Inverted | Blue, Green |
and Red |
. Cyan and Yellow available on request. | Greyscale ultra-high sensitivity EMCCD | Optimally configured for high resolution and high sensitivity fluorescence microscopy of living specimens in XYZT for deconvolution, TIRF and super-resolution using N-STORM and dSTORM. |