...
Microscope Name | Upright or Inverted? | Laser Lines | Features | Live Imaging | Techniques |
|---|---|---|---|---|---|
SPE | Upright | 405, 488, 532, 640 | Leica TCS SPE with DM2500 stand. 1x PMT detector. DIC imaging. | Perfusion chamber and dipping lenses on request. | 3D imaging of fixed specimens on slides. Live imaging possible but not fast. Orthogonal (XZ) scans possible with the Z Galvo. |
SPE2 | Upright | 405, 488, 532, 640 | Leica TCS SPE with DM2500 stand. 1x PMT detector. DIC imaging. | Oko-Lab stage adapter and dipping lenses on request. | 3D imaging of fixed specimens on slides. Live imaging possible but not fast. Orthogonal (XZ) scans possible with the Z Galvo. |
SPE3 | Inverted | 405, 488, 561, 640 | Leica TCS SPE with DMI4000 stand. 1x PMT detector. DIC imaging. Motorised stage. Matrix screener module. | No incubation equipment is available for this microscope. | 3D imaging of fixed specimens in slides and dishes. Fluorescence and DIC on grids for CLEM. Multi-position imaging and tiling. Matrix Screener for high content screening applications. |
SP5 | Inverted | 405, 457, 476, 488, 495, 561, 594, 633 | Leica TCS SP5 with DMI6000B stand. 3x Hybrid and 1x PMT decectorsdetectors. DIC. Motorised stage. FRAP and FRET wizards. Live Data Mode and Matrix Screener. | Pe-Con stage-top incubator for 35 mm dishes. | 4D (3D+T) imaging of live and fixed specimens. CLEM. Multi-position acquisition and tiling. FRAP and FRET Acceptor Photobleaching or Sensitised Emission. Complex experiment design. |
| Olympus FV1200 | Inverted | 405, 458, 488, 515, 559, 635. Sim Scanner: 405 (pulsed for ablation), 440 | Olympus FV1200 with IX83 stand. 3x PMT, 2x GaAsP detectors. Second scanner for simultaneous imaging and ablation. Motorised stage. Silicon lenses. Time Controller module. | Tokai-Hit stage-top incubator with adaptors for dishes and chambered coverslips. | 4D (3D+T) imaging of live and fixed specimens. High sensitivity imaging of green and red dyes with GaAsP detectors. Ablation of actin cortex. CLEM. Multi-position acquisition and tiling. Imaging thicker specimens. Complex experiment design. |
| Multiphoton | Inverted | 405, 458, 488, 514, 561, 633. Tune-able Ti-Sapphire and OPO | Zeiss LSM 880 NLO with Axio Observer Z1 stand. 32 channel GaAsP, 2x PMT, 2x NDD and BiG2. Airyscan. Motorised stage. FCS/FCCS. Experiment Designer. | Pe-Con cage incubator with CO2 chamber for 35 mm dishes | 4D (3D+T) imaging of live and fixed specimens. Multiphoton imaging and ablation. Fast spectral imaging and un-mixing. Super-resolution. Multi-position acquisition. Complex experiment design. |
Radiance | Upright | 458, 488, 514, 543, 635 | Bio-Rad radiance 2100 with Nikon E800 stand. 3x GaAsP detectors. Transmitted light contrast by DIC. Basic FRAP capability. | Tokai-Hit stage heater and Bioptechs objective lens heater available on request. | 3D imaging of fixed specimens on slides. |
...
| Microscope | Upright or Inverted | Laser Lines | Key Features | Live Imaging | Techniques |
|---|---|---|---|---|---|
| Elyra | Inverted | 405, 488, 561, 642 | Zeiss Elyra PS.1 with Axio Observer Z1 stand. Andor iXon Du-897 EMCCD for PALM. PCO Edge sCMOS for SIM. Motorised stage and piezo focus. Single-band filter turret and optional dual-band filter turret for high speed dual channel imaging. 3D SIM and 3D PALM capabilities. | Tokai-Hit stage-top incubator with adaptors suitable for 35 mm dishes and chambered coverslips. | Structured Illumination Microscopy, PALM and dSTORM, Total Internal Reflection (TIRF) microscopy |
| GFP4 | Inverted | 405, 488, 561, 642 | Visitech vt-iSIM and Nikon TiE stand. Hamamatsu Orca Flash4.0 v3 camera for vt-iSIM. Andor Zyla for wide-field imaging. Motorised stage and piezo focus. | Oko-Lab cage incubator with adaptors for multi-well plates, 35 mm dishes and chambered coverslips. | Instant SIM that gives a live structured illumination image without processing. Further resolution possible with deconvolution. Wide-field. |
| N-STORM | Inverted | 405, 488, 561, 647 | Nikon N-STORM and Ti stand. Andor iXon Du-897 EMCCD camera for STORM and dSTORM. Astigmatic lens for 3D STORM. Zyla sCMOS camera for wide-field imaging. | Solent Scientific cage incubator with adaptors for multi-well plates and 35 mm dishes. | Stochastic Optical Reconstruction Microscopy (STORM) and direct STORM. TIRF microscopy. Wide-field time-lapse. |
| STED | Inverted | 405, WLL | Leica TCS gSTED 3X with DMI6000B stand. 3x Hybrid and 2x PMT detectors. Motorised stage. High speed Z Galvo focus. 532, 660 and 775 nm depletion lasers for multi-colour STED. 3D STED capability. | Life Imaging Services Box cage incubator system with adaptors for 35 mm dishes and chambered coverslips | Multi-colour STED and confocal microscopy. 3D STED. Tiling of large specimens. |