It might be necessary to correct for chromatic shift in images and stacks if you are acquiring data for colocalisation analysis. Volocity can generate Registration Corrections that can be applied to 3D data to shift pixels in all channels back to their correct positions.
Creating and Using Registration Corrections
Use a suitable specimen to create a registration correction. I use a slide of 0.5 micron multicolour beads because they are small, bright and are labelled with blue, green, red and far red fluorophores. Capture 3D stacks of these beads at the same XY and Z resolution you will be acquiring your data at so that the pixel shift is correct.
...