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1. Double click on the LAS X desktop icon to start the Leica acquisition software.
   
   
   
   
2. A Configuration pop-up window opens. Select Configuration - machine.xlhw, Microscope – DMI6000 and Resonant – OFF (unless you want to use the resonant scanner for fast imaging).
   
   1. For the confocal only mode select STED – OFF
   2. For the STED mode select STED – ON
      

   Press OK.
   
   
   
   

3. When the Microscope Stand pop-up window appears make sure that the condenser is all the way up, so that it does not interfere with the moving stage, then select YES). 
   
   
   
   
4. After some time the start screen of the software will appear and you are ready to start setting up your experiment.
   
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