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Make sure the camera is on before opening µManager. If you're using Windows then double-click the µManager icon on the desktop. If you're using a Mac then you can click its icon in the dock. Note that the icon may say 'ImageJ' when you hover the mouse over it - that's normal.
µManager configuration
Select the correct configuration from the drop-down list that appears with the splash screen. The image below shows configuration for the QIClick on the Axioplan microscope. If the configuration you want isn't listed you can browse for it by clicking the button to the right of the list. If you want to process data without turning the camera on you can select '(none)' from the list.
µManager user interface
The image below shows a typical µManager GUI. The details of the Groups and Presets may vary from system to system. Select the right Magnification Preset to match the objective lens and optovar (if applicable). Make sure the camera depth is set to its maximum (usually 12-bit). Click Live to get a live image.
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Click the Zoom tools to make the live image bigger or smaller. Set the exposure by typing a value in the field. Change the binning when necessary. There may also be a gain control. You can click the Auto button next to the live image contrast histogram to auto-contrast the image once, or you can tick Autostretch to have auto contrast on constantly.
Image acquisition using the Album button
The best way to acquire images is click the Album button at the bottom of the live image window (see below). This creates a new window containing the image you just captured. As you keep clicking the button the images are added to the window and can be saved either as a stack or individual TIFF files by clicking the disc icon.
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