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The best way to acquire images is click the Album button at the bottom of the live image window (see below). This creates a new window containing the image you just captured. As you keep clicking the button the images are added to the window and can be saved either as a stack or individual TIFF files by clicking the disc icon. It's best if you save the image as a stack if possible because this will open correctly when you use the Bioformats Importer in Fiji or ImageJ. Otherwise you can make a copy of the data using the ImageJ Duplicate... command in the Image menu, but you will need to fill in the number of slices in the Range field.

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Live image album and save buttons

The bottom of the Live window with Save icon (left) and Album (right)