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The image on the right shows a typical µManager GUI. The details of the Groups and Presets may vary from system to system. Select the right Magnification Preset to match the objective lens and optovar (if applicable). Make sure the camera depth is set to its maximum (usually 12-bit). Click Live to get a live image. Click the Zoom tools to make the live image bigger or smaller. Set the exposure by typing a value in the field and change the binning if necessary. There may be a gain control. Click the Auto button next to the live image contrast histogram to auto-contrast the image once, or you can tick Autostretch to have auto contrast on constantly (see below).

The best way to acquire images is click the Album button at the bottom of the live image window (see below). This creates a new window containing the image you just captured. As you keep clicking the button the images are added to the window and can be saved either as a stack or individual TIFF files by clicking the disc icon. It's best if you save the image as a stack if possible because this will open correctly when you use the Bioformats Importer in Fiji or ImageJ. Otherwise you can make a copy of the data in ImageJ using the Duplicate... command in the Image menu, but you will need to fill in the number of slices in the Range field.