I would recommend saving data in Openlab's native LIFF format initially so all image metadata (including calibrations) is retained. Openlab LIFF files (not to be confused with Leica's LIF confocal file format) are compatible with the Bioformats Importer so you should be able to open them in Fiji provided all the images in the file are at the same depth. You can also save in other formats like TIFF but the image metadata will not be retained so you will have to make a note of the image calibration. TIFF for Publication is a spacial format that saves the data at a depth of RGB colour regardless of what it was captured at. This is so images captured at unusual depths like 12-bit render correctly in basic image viewers like Mac Preview and Windows Photo Viewer as well as PowerPoint. TIFF for Publication images should not be used for quantitation. | 