I would strongly recommend saving data in Openlab's native LIFF format initially so all image metadata is retained and no image data is lost. Openlab LIFF files (not to be confused with Leica's LIF confocal file format) are compatible with the Bioformats Importer so you should be able to open them in Fiji provided all the images in the file are at the same depth. You can also save in other formats like TIFF but the image metadata will not be retained so you will have to make a note of the image calibration. TIFF for Publication is a special format that saves the data at a depth of RGB colour regardless of what it was captured at. This is so images captured at unusual depths like 12-bit render correctly in basic image viewers like Preview and Windows Photo Viewer as well as in PowerPoint. TIFF for Publication images are for presentation only and should not be used for quantification. 'Save As Multiple...' saves layers separately rather than as a single layered file. You might find this useful if you want to save images as TIFFs to further process in photo editing software because Photoshop and GIMP don't support layers in the same way as scientific processing software. You must to select the layers you want to save and you must define a Naming scheme for the images (e.g. by telling Openlab to sequentially number them). | 