...
OBJECTIVE LENSES | |||||
|---|---|---|---|---|---|
Objective | Magnification | Immersion Medium | Numerical Aperture | Coverslip Correction [mm] | Working Distance [mm] |
CFI Plan Fluor 10x | 10x | air | 0.3 | 0.17 | 16 |
CFI Plan Fluor 40x oil | 40x | oil | 1.3 | 0.17 | 0.2 |
CFI Plan Apo VC 60x WI | 60x | water | 1.2 | 0.15 – 0.18 | 0.31 – 0.28 |
CFI Plan Apo VC 60x oil | 60x | oil | 1.4 | 0.17 | 0.13 |
CFI Apo TIRF 100X Oil | 100x | oil | 1.49 | 0.13 – 0.19 (23°C) 0.14 (37°C) | 0.12 |
A
...
FLUORESCENCE FILTER SETS | ||
|---|---|---|
Turret Position | Emission Filter | Example Fluorophores |
1 | 49000 | DAPI |
2 | 49002 | GFP |
3 | 49008 | mCherry |
4 | C-S2 | Quadband compatible with all Lumencore lines |
5 | C-STO | Quadband compatible with all Agilent laser lines |
6 | N-STO | Alexa Fluor 647, Cy5 |
A
...
CAMERA/DETECTOR |
|---|
Andor iXon DU897E (EMCCD) for STORM imaging (right side port) |
Andor Technology sCMOS Zyla 4.2 for widefield acquisitions |
A
...
INCUBATION
...
: Solent Scientific chamber heater and CO2 supply
...
unit
...
SOFTWARE
...
: NIS-Elements Ar including NIS-A N-STORM analysis module
...
ADDITIONAL INFORMATION |
|---|
anti-vibration optical table |
fully enclosed |
perfect focus system (PFS) |
motorized XY stage with encoders |
piezo Z stage |