Lasers
| Excitation Lasers | ||
|---|---|---|
| Wavelength | Type | Fluorophores |
| 405 nm | CW | DAPI, Hoechst, etc |
| White Light Laser | Pulsed | All visible dyes from green to far red |
| STED Depletion Lasers | ||
| 592 nm | CW | STED with green dyes |
| 660 nm | CW | STED with red dyes |
| 775 nm | Pulsed | STED with far red dyes |
MICROSCOPE: DMI6000, inverted
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STED RESOLUTION
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XY
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~ 50 nm
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Z
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same as confocal
IMAGING MODES:
- Laser scanning confocal imaging (multi-colour, multi-point, live cell)
- STED with 592 nm depletion, up to 3 colours
A
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OBJECTIVE LENSES
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Objective
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Magnification
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Immersion Medium
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Numerical Aperture
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Coverslip correction [mm]
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Working Distance [mm]
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Objective lenses
| Objective lenses | |||||
|---|---|---|---|---|---|
| Magnification | Type | Immersion | NA | Working Distance (mm) | Coverslip correction |
| 10x | HC PL FLUOTAR | Air | 0.3 | 11 | 0.17 |
| 20x | HC PL APO CS2 | ||||
| Multi | 0.75 |
| 0.68 |
| 0/0. |
| 17 | |
| 25x | HC FLUOTAR L VISIR |
| Water |
| 0.95 | 2.4 | 0.17 |
| 40x | HC PL APO CS2 |
| Oil | 1.3 | 0. |
| 24 | 0. |
| 17 | |
| 63x | HC PL APO CS2 /1.4 (WD = 0.14 mm) |
| Oil | 1.4 | 0. |
| 14 | 0. |
| 17 | |
| 100x | HC PL APO CS2 (dedicated STED objective) |
| Oil | 1.4 | 0. |
| 13 | 0. |
| 17 |
NOTE: There There is only space for 6 objective lenses on the objective turret, so if the lens you would like to use is not on there please contact contact lmcb-lm-help@ucl.ac.uk
MICROSCOPE: DMI6000, inverted
...
STED RESOLUTION | |
|---|---|
XY | ~ 50 nm |
Z | same as confocal |
...
IMAGING MODES:
- Laser scanning confocal imaging (multi-colour, multi-point, live cell)
- STED with 592 nm depletion, up to 3 colours
...
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FLUORESCENCE FILTER SETS: This microscope uses an Acousto-Optical Beam Splitter (AOBS) with a freely selectable emission detection range rather than fluorescence emission filter cubes.
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