Lasers
| Excitation Lasers | ||
|---|---|---|
| Wavelength | Type | Fluorophores |
| 405 nm | CW | DAPI, Hoechst, etc |
| White Light Laser† | Pulsed | All visible dyes from green to far red |
| STED Depletion Lasers‡ | ||
| 592 nm | CW | STED with green dyes |
| 660 nm | CW | STED with red dyes |
| 775 nm | Pulsed | STED with far red dyes |
† The White Light Laser (WLL) is a super-continuum that can be tuned from 470 nm to 670 nm and can therefore be used to excite all visible dyes.
‡ The depletion lasers are used to increase resolution by stimulating re-emission of photons. They are not used for excitation and are only used in STED.
Objective lenses
| Objective lenses | |||||
|---|---|---|---|---|---|
| Magnification | Type | Immersion | NA | Working Distance (mm) | Coverslip correction |
| 10x | HC PL FLUOTAR | Air | 0.3 | 11 | 0.17 |
| 20x | HC PL APO CS2 | Imm (Oil, Glycerol and Water) | 0.75 | 0.68 | 0/0.17 |
| 25x | HC FLUOTAR L VISIR | Water | 0.95 | 2.4 | 0.17 |
| 40x | HC PL APO CS2 | Oil | 1.3 | 0.24 | 0.17 |
| 63x | HC PL APO CS2 | Oil | 1.4 | 0.14 | 0.17 |
| 100x | HC PL APO CS2 (dedicated STED objective) | Oil | 1.4 | 0.13 | 0.17 |
NOTE: There is only space for 6 objective lenses on the objective turret, so if the lens you would like to use is not on there please contact lmcb-lm-help@ucl.ac.uk
MICROSCOPE: DMI6000, inverted
STED RESOLUTION | |
|---|---|
XY | ~ 50 nm |
Z | same as confocal |
IMAGING MODES:
- Laser scanning confocal imaging (multi-colour, multi-point, live cell)
- STED with 592 nm depletion, up to 3 colours
FLUORESCENCE FILTER SETS: This microscope uses an Acousto-Optical Beam Splitter (AOBS) with a freely selectable emission detection range rather than fluorescence emission filter cubes.
DETECTORS | |
|---|---|
3x HyD, high sensitivity, 3 operating modes (standard, counting, BrightR) + time-gating | |
3x PMT, one of which for transmitted light imaging | |
A
INCUBATION: The microscope is fully enclosed and equipped with The Cube chamber heater and The Brick CO2 gas mixer.
SOFTWARE | |
|---|---|
Acquistion | LAS X |
Deconvolution | Huygens Professional Version 15.10.1p2 |
A
ADDITIONAL INFORMATION:
- resonant scanner (8000 Hz) for fast imaging
- anti-vibration optical table
- fully enclosed
- autofocus
- motorized XY stage
- Z-galvo