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NOTE: There is only space for 6 objective lenses on the objective turret, so if the lens you would like to use is not on there please contact lmcb-lm-help@ucl.ac.ukFLUORESCENCE FILTER SETS: This microscope uses an Acousto-Optical Beam Splitter (AOBS) with a freely selectable emission detection range rather than fluorescence emission filter cubes.
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Fluorescence Filter Sets
| Name | Excitation Filter | Dichroic Mirror | Emission Filter | Typical Fluorophores |
|---|---|---|---|---|
| I3 | BP 450-490 | 510 | LP 515 | FITC, GFP, Alexa 488 |
| N2.1 | BP 515-560 | 580 | LP 590 | TRITC, RFP, Alexa 568 |
| Empty | - | - | - | - |
| BF | Empty | Empty | Empty | Confocal or Brightfield |
| A | BP 340-380 | 400 | LP 425 | DAPI, Hoechst |
| - |
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DETECTORS | |
|---|---|
3x HyD, high sensitivity, 3 operating modes (standard, counting, BrightR) + time-gating | |
3x PMT, one of which for transmitted light imaging | |
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