...
The microscope is a fully motorised DMI 6000 CS inverted model with AFC auto focus, a Märzhäuser motorised scanning stage and a high speed Z Galvo focus in addition to the stand's internal focus motor. Differential Interference Contrast (DIC) is available for the 10x, 20x and 40x lenses. Fluorescence can be viewed down the eyepieces using the EL6000 metal halide lamp and the filter sets below.
| Note |
|---|
The EL6000 lamp and filters in the table below are only for viewing the specimen down the eyepieces. The confocal/STED imaging system users lasers to illuminate the specimen and a spectral detection system to filter fluorescence. |
| Filter sets | ||||
|---|---|---|---|---|
| Name | Excitation Filter | Dichroic Mirror | Emission Filter | Typical Fluorophores |
| I3 | BP 450-490 | 510 | LP 515 | FITC, GFP, Alexa 488 |
| N2.1 | BP 515-560 | 580 | LP 590 | TRITC, RFP, Alexa 568 |
| POL | - | - | - | Polariser for DIC |
| BF | Empty | Empty | Empty | Confocal or Brightfield |
| A | BP 340-380 | 400 | LP 425 | DAPI, Hoechst |
| Empty | - | - | - | Spare position |
...
...
DETECTORS | |
|---|---|
3x HyD, high sensitivity, 3 operating modes (standard, counting, BrightR) + time-gating | |
3x PMT, one of which for transmitted light imaging | |
...