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- RC-37F insert for Falcon 35mm dishes (P/N 64-0348)
- MDA-1 mini-dish adapter for RC-20 series (P/N 64-1675)
- SA-20-MW-AL stage adapter for multi-wells (P/N 64-2416)
- TY-50 Tygon 1/10” tubing (P/N 64-0167)
- TC-3 tubing connectors (P/N 64-0164)
- SL-6 Luer stopcocks (P/N 64-0165)
Warner Instruments RC-37F - perfusion apparatus with syringe manifold
Figure 1A: This image illustrates the overall appearance of a syringe manifold for supplying fluid to the RC-37F insert for 35 mm dishes. The flow is controlled using a pinch valve assembly that is controlled by switches on a VC-6 six channel valve controller.
Figure 1B: A 50 ml syringe is mounted in the syringe manifold and connected to TY-50 Tygon 1/10” tubing by an SL6 Luer stopcock connected to a tubing adapter (TC-3)
Figure 1C: A short piece of C-FLEX tubing is inserted into one of the pinch valves. This C-FLEX tubing is connected above and below the valve to lengths of TY-50 Tygon 1/10” tubing with tubing adapters (TC-3).
Figure 1D: The RC-37F insert is mounted in a 35 mm Mat-Tek glass-bottomed dish. The dish is mounted in an MDA-1 mini-dish adapter for the RC-20 series of Warner chambers. The MDA-1 clamps are used to hold the RC-37F in the dish. The MDA-1 is further mounted in an SA-20-MW-AL stage adapter for multi-wells, which will fit in any microscope specimen adapter designed for multi-well plates. The RC-37F inlet port is connected to an MP-2 manifold using short pieces of PE-160 polyethylene tubing. The outlet port is attached to a short piece of C-FLEX tubing. Warner Instruments recommend attaching the outlet to a vacuum line, but a peristaltic or syringe pump might also work.