Warner Instruments perfusion equipment

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The LMCB light microscopy facility has several items of equipment from Warner Instruments for perfusion and environmental control of chambers inserted into 35 mm dishes. The information below should hopefully provide simple guides to assembling the equipment for use in microscope environmental chambers. There are also detailed manuals for the individual items of equipment that are stored with the equipment itself. More information about the equipment and consumables that are available from Warner Instruments can be found at the following website: Warner Instruments

Warner Instruments RC-37F - perfusion apparatus with two syringes

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The RC-37F inlet and outlet ports are connected to short lengths of TY-50 Tygon 1/10” tubing which are in turn connected by TC-3 tubing connectors to SL-6 Luer stopcocks and standard plastic syringes. The TY-50 tubing can be cut to any length.

Warner Instruments equipment and consumables

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• RC-37F insert for Falcon 35mm dishes (P/N 64-0348)
• MDA-1 mini-dish adapter for RC-20 series (P/N 64-1675)
• SA-20-MW-AL stage adapter for multi-wells (P/N 64-2416)
• TY-50 Tygon 1/10” tubing (P/N 64-0167)
• TC-3 tubing connectors (P/N 64-0164)
• SL-6 Luer stopcocks (P/N 64-0165)

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D. The RC-37F insert is mounted in a 35 mm Mat-Tek glass-bottomed dish. The dish is mounted in an MDA-1 mini-dish adapter for the RC-20 series of Warner chambers. The MDA-1 clamps are used to hold the RC-37F in the dish. The MDA-1 is further mounted in an SA-20-MW-AL stage adapter for multi-wells, which will fit in any microscope specimen adapter designed for multi-well plates. The RC-37F inlet port is connected to an MP-2 manifold using short pieces of PE-160 polyethylene tubing. The outlet port is attached to a short piece of C-FLEX tubing. Warner Instruments recommend attaching the outlet to a vacuum line, but a peristaltic or syringe pump might also work.

Warner Instruments equipment and consumables:

• RC-37F insert for Falcon 35mm dishes (P/N 64-0348)
• MDA-1 mini-dish adapter for RC-20 series (P/N 64-1675)
• SA-20-MW-AL stage adapter for multi-wells (P/N 64-2416)
• TY-50 Tygon 1/10” tubing (P/N 64-0167)
• TC-3 tubing connectors (P/N 64-0164)
• SL-6 Luer stopcocks (P/N 64-0165)
• CFL-6 C-FLEX tubing 1/32" (P/N 64-0166)
• PE-160 tubing, 1.57 OD x 1.41 ID (P/N 64-0755)
• MP-2 manifold (P/N 64-0206)
• VC-6 six channel valve controller with pinch valve assembly
• SH-6/60 Syringe holder with six 60 cc syringes (P/N 64-0163)