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| Light source | Type | Wavelengths [nm] |
| Diode | Laser | 405, 440 |
| Diode | Laser | 559 |
| Diode | Laser | 635 |
| Multi-line Argon ion | Laser | 458, 488. 515 |
| Ablation laser | Laser (pulsed or cw) | 405 |
| U-HGLGPS | Mercury metal halide bulb | ~ 300 - 700 (peaks at 365, 405, 436, 546, 579) |
A
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OBJECTIVE LENSES
| Objective | Magnification | Immersion Medium | Type | Numerical ApertureNA | Coverslip correction [mm] | Working Distance WD [mm] | |
|---|---|---|---|---|---|---|---|
| UPLSAPO10X2 | 10x | air | UPLSAPO10X2 | 0.4 | 0.17 | 3.1 | |
| UPLSAPO20X | 20x | air | UPLSAPO20X | 0.75 | 0.17 | 0.6 | |
| UPLFN40X0 | 40x | oil | UPLFN40X0 | 1.3 | 0.17 | 0.2 | |
| PLAPON60X0SC2 | 60x | oil | PLAPON60X0SC2 | 1.4 | 0.17 | 0.12UPLSAPO60XS | |
| 60x | silicone oil | UPLSAPO60XS | 1.3 | 0.15 - 0.19 | 0.3 | ||
| UPLSAPO30XS | 30x | silicone oil | UPLSAPO30XS | 1.05 | 0.13 - 0.19 | 0.8 | The objective lens below is also available for this microscope|
| UPLSAPO60XW | 60x* | water | UPLSAPO60XW | 1.2 | 0.13 - 0.21 | 0.28 |
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NA = Numerical Aperture, WD = Working Distance |
There is also a 60x UPLSAPO60XW water lens with NA 1.2, WD 0.28 and a correction collar for coverslip thicknesses 0.13 to 0.21 mm. It is kept in a drawer because there isn't a free position in the nose piece. If you need to use this leans then please ask a member of the Light Microscopy Facility staff because the microscope software settings must be edited when an objective lens changes.
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FLUORESCENCE FILTER SETS: Filter sets for imaging a wide range of standard visible and far-red fluorophores are available.
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