Olympus FluoView FV1200 - Technical Specifications

MICROSCOPE: IX83, inverted

IMAGING MODES

Laser scanning confocal imaging (multi-colour, multi-point, live cell time-lapse, Z-stack)
FRET
FRAP
FLIP
λ-scan
uncaging

LIGHT SOURCES

Light source	Type	Wavelengths [nm]
Diode	Laser	405, 440
Diode	Laser	559
Diode	Laser	635
Multi-line Argon ion	Laser	458, 488. 515
Ablation laser	Laser (pulsed or cw)	405
U-HGLGPS	Mercury metal halide bulb	~ 300 - 700 (peaks at 365, 405, 436, 546, 579)

A

OBJECTIVE LENSES

Magnification	Immersion	Type	NA	Coverslip correction [mm]	WD [mm]
10x	air	UPLSAPO10X2	0.4	0.17	3.1
20x	air	UPLSAPO20X	0.75	0.17	0.6
40x	oil	UPLFN40X0	1.3	0.17	0.2
60x	oil	PLAPON60X0SC2	1.4	0.17	0.12
60x	silicone oil	UPLSAPO60XS	1.3	0.15 - 0.19	0.3
30x	silicone oil	UPLSAPO30XS	1.05	0.13 - 0.19	0.8
60x*	water	UPLSAPO60XW	1.2	0.13 - 0.21	0.28
NA = Numerical Aperture, WD = Working Distance

There is also a 60x UPLSAPO60XW water lens with NA 1.2, WD 0.28 and a correction collar for coverslip thicknesses 0.13 to 0.21 mm. It is kept in a drawer because there isn't a free position in the nose piece. If you need to use this leans then please ask a member of the Light Microscopy Facility staff because the microscope software settings must be edited when an objective lens changes.

FLUORESCENCE FILTER SETS: Filter sets for imaging a wide range of standard visible and far-red fluorophores are available.

DETECTORS

3 channel PMT (Hamamatsu R7862)
External transmitted light PMT (Hamamatsu R7400)
Ultra-high sensitivity detector GaAsP with onboard Peltier cooling system (2 channel)

INCUBATION: TokaiHit CO₂ stage top incubator including lens heater

SOFTWARE: FV10-ASW 4.2

ADDITIONAL INFORMATION

anti-vibration optical table
motorized Z-drift compensator (ZDC)
motorized XY stage
Silver coated galvo scanner
SIM scanner for simultaneous photostimulation and confocal image acquisition