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- Start the microscope as usual, but without turning on the software
- Turn on the EPI fluorescence lightpath and focus on your sample using the ocular (e.g. 1 μm TetraSpeck beads)
- Adjust the lens correction collar for the immersion medium, the cover glass thickness and temperature you are using, if the lens has one before turning on the ZDC (not all lenses are compatible with ZDC, for list see below)
- To adjust the Near Limit Setting for the objective lens you are using go to the Settings menu by pressing the spanner icon on the touch panel controller
- Select Customized -> Focus Limit Setting
- Look down the eyepiece and focus slightly above your sample
- To set the Z-position you are now in as Z-Focus Limit press Set and then OK on the touch panel controller
- Close the Settings menu
- Make sure that the Z-drift compensation port switching lever (on the left under the stage) is in the IN position. This engages the dichroic mirror in the light path of the Z-drift compensation sensor unit and therefore enables Z-drift correction
- Check that the DIC slider (on the right under the objective turret) is removed from the lightpath (pull out)
- Open the FV10-ASW 4.2 acquisition software
- Setup the light path, start a live preview and check the sample is in focus
- Make sure to set up a time series as ZDC is only available in the time series mode
- Click ZDC -> ZDC Settings in the software
