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Before adjusting for Köhler you need to know where the relevant condenser components are on the microscope. This varies between manufacturers and models. The image below gives some examples of where to find the field diaphragm, iris aperture, etc. If in doubt, please as a member of light microscopy staff.
The condensers of various upright and inverted microscopes. The irises and other components can be located differently depending on the manufacturer and model.
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