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Before adjusting for Köhler you need to know where the relevant condenser components are on the microscope. This varies between manufacturers and models. The image below gives some examples of where to find the field diaphragm, iris aperture, etc. If in doubt, please as a member of light microscopy staff.

Condensers of various microscopesImage RemovedCondensers of various microscopesImage Added

The condensers of various upright and inverted microscopes. The irises and other components can be located differently depending on the manufacturer and model.

  1. Field diaphragm. The DM2500 field diaphragm is on the other side of the stand. The Nikon Ti field diaphragm is near the top of the transmitted light pillar, labelled F
  2. Condenser focus
  3. Centring screws. The Leica DM2500 and DMI6000 have to be centred using hexagonal screwdrivers or Allen keys
  4. Iris aperture. The iris aperture on the DMI6000 is adjusted using the AP buttons on the left of the stand

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